Analysis of the assembly apparatus of photosystem I reaction center complex

2004 Fiscal Year Final Research Report Summary

• Project/Area Number: 15570037
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 植物生理・分子
• Research Institution: Okayama University
• Principal Investigator: TAKAHASHI Yuichiro Okayama University, Faculty of Science, Biology, Professor, 理学部, 教授 (50183447)
• Project Period (FY): 2003 – 2004
• Keywords: photosynthesis / photosystem / assembly / chloroplast / transformation / Ycf4 / tag / affinity chromatography

Research Abstract

Chloroplast-encoded Ycf4 is a thylakoidal protein and is essential for the assembly of photosystem I (PSI) reaction center complex. This protein is a part of a large complex that is an assembly apparatus of PSI complex. In this project, the Ycf4-complex was highly purified by two steps affinity chromatography from the thylakoid extracts of the chloroplast transformant of Chlamydomonas reinhardtii in which Ycf4 fused with TAP-tag is expressed. The resultant Ycf4-complex was found to contain Cop2, the retinal binding protein, and MASAP (mitochondrial ATP synthase associated protein) as well as PsaF, one of the PSI subunits and Ycf4. It was found from separate biochemical experiments that all Cop2 present in the thylakoids is co-purified with Ycf4, indicating that this retinal binding protein is an authentic component of the Ycf4-complex. The functional role of Cop2 is not yet known. Absorption and fluorescence emission spectra revealed that the purified Ycf4-complex contains chlorophyll and carotenoid. These pigments may be supplied to an assembly intermediate of PSI complex.

Research Products

• [Journal Article] The Light-harvesting complex of photosystem 1 in Chlamydomonas reinhardtii : Protein composition, gene structures and phylogenic implications (2004)
  • Author(s): Tokutsu R, Teramoto H, Takahashi Y, Ono T, Minagawa J
  • Journal Title: Plant and Cell Physiology 45 Pages: 138-145
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Comparison of the subunit compositions of the PSI-LHCI supercomplex and the LHCI in the green alga Chlamydomonas reinhardtti (2004)
  • Author(s): Y.Takahashi, T.Yasuil, E.J.Stauber, M.Hippler
  • Journal Title: Biochemistry 43 Pages: 7816-7823
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Post-transcriptional steps involved in the assembly of photosystem I in Chlamydomonas (2004)
  • Author(s): J.-D.Rochaix, K.Perron, D.Dauvillee, F.Laroche, Y.Takahashi, M.Goldschmidt
  • Journal Title: Biochemical Society Transactions 32 Pages: 567-570
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Localization and function of small chloroplast-encoded PsbT polypeptide of photosystem II in the green alga Chlamydomonas reinharrdtii (2004)
  • Author(s): N.Ohnishi, Y.Takahashi
  • Journal Title: Endocytobiosis Cell Research 15 Pages: 181-188
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Structure, function and assembly of Photosystem II and its light-harvesting proteins (2004)
  • Author(s): J.Minagawa, Y.Takahashi
  • Journal Title: Photosynthesis Research 82 Pages: 241-263
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Establishment of publicly available cDNA material and information resource of Chlamydomonas reinhardtii (Chlorophyta) to facilitate gene function analysis. (2004)
  • Author(s): Asamizu E,......, Takahashi Y, Tabata S
  • Journal Title: Phycologia 43 Pages: 722-726
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The Light-harvesting complex of photosystem I in Chlamydomonas reinhardtii : Protein composition, gene structures and phylogenic implications. (2004)
  • Author(s): Tokutsu R, Teramoto H, Takahashi Y, Ono T, Minagawa J
  • Journal Title: Plant Cell Physiol. 45 Pages: 138-145
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Comparison of the subunit compositions of the PSI-LHCI supercomplex and the LHCI in the green alga Chlamydomonas reinhardtti (2004)
  • Author(s): Y.Takahashi, T.Yasui1, E.J.Stauber, M.Hippler
  • Journal Title: Biochemistry 43 Pages: 7816-7823
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Post-transcriptional steps involved in the assembly of photosystem I in Chlamydomonas (2004)
  • Author(s): J.-D.Rochaix, K.Perron, D.Dauvillee, F.Laroche, Y.Takahashi, M.Goldschmidt-Clermont
  • Journal Title: Biochemical Society Transactions 32 Pages: 567-570
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Localization and function of small chloroplast-encoded PsbT polypeptide of photosystem II in the green alga Chlamydomonas reinharrdtii (2004)
  • Author(s): N.Ohnishi, Y.Takahashi
  • Journal Title: Endocytobiosis Cell Res. 15 Pages: 181-188
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Structure, function and assembly of Photosystem II and its light-harvesting proteins (2004)
  • Author(s): J.Minagawa, Y.Takahashi
  • Journal Title: Photosynthesis Res. 82 Pages: 241-263
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Establishment of publicly available cDNA material and information resource of Chlamydomonas reinhardtii (Chlorophyta) to facilitate gene function analysis. (2004)
  • Author(s): Asamizu E, Nakamura Y, Miura K, Fukuzawa H, Fujiwara S, Hirono M, Iwamoto K, Matsuda Y, Minagawa J, Shimogawara K, Takahashi Y, Tabata S
  • Journal Title: Phycologia 43 Pages: 722-726
  • Description: 「研究成果報告書概要(欧文)」より