Reconstitution of lypophilic components in Photosystem I complex which had been extracted with diethyl ether

2005 Fiscal Year Final Research Report Summary

• Project/Area Number: 15570040
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 植物生理・分子
• Research Institution: Teikyo University
• Principal Investigator: IKEGAMI Isamu Teikyo Univ., Fac.Pharm.Sci., Prof., 薬学部, 教授 (10082322)
• Project Period (FY): 2003 – 2005
• Keywords: Photosynthesis / Photosystem I / Photosynthetic Reaction center / Chlorophyll / P700

Research Abstract

1.Chlorophylls, such as Chl b, Chl a epimer and Phe a, all which do not act as intrinsic antenna in the PSI RC complex, were added with phosphatidylglycerol to the antenna-depleted PSI complex that had a Chl a/P700 ratio of 12. We found that the Chl a-binding site allows to receive Chl b with the same affinity as Chl a, but Chl a epimer with the affinity half of Chl a. The bound Chl b and Chl a epimer act as efficient antenna as Chl a. Phe a binds the antenna-depleted PSI complex, but without any efficient transfer of light energy to P700. We conclude that Chl a-binding site is tolerable to Chl with a side-chain different from Chl a, showing lower affinity with a large structural difference, and that Mg is essential to bind the site.
2.Chlorella, when grown in the dark with an extremely high concentration of Zn, formed Zn-Chl a in amount of about 30% of Chl a. We found that the Zn-Chl a binds to PSI and PSII RC complexes and acts as efficient antenna as Mg-Chl a.
3.PSI complex isolated from phylloquinone-depleted mutant of Arabidopsis, had no P700 but emitted the far red fluorescence at 77K which is characteristic to PSI antenna. We suggest that it had a PSI complex with antenna Chl a but without PSI electron carriers such as P700 and A_0.

Research Products

• [Journal Article] Energy transfer and charge separation dynamics in Chlorophyll d-based photosystem I from Acaryochloris marina (2005)
  • Author(s): Kumazaki, S., et al.
  • Journal Title: Photosynthesis : Fundamental Aspects to Global Perspectives Pages: 92-94
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The energy gap between P700^+-A_0^- and P700^* in photosystem I determined by delayed fluorescence at 270-77K (2005)
  • Author(s): Shibata, Y., et al.
  • Journal Title: Photosynthesis : Fundamental Aspects to Global Perspectives Pages: 94-97
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Zn-Chl a is accumulated functionally in Chlorella hetero-trophycally grown in the dark with an excessive amount of Zn^<2+> (2005)
  • Author(s): Ikegami, I., et al.
  • Journal Title: Photosynthesis : Fundamental Aspects to Global Perspectives Pages: 157-158
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Inactivation an defficiency of core proteins of photosystems I and II caused by genetical phylloquinone and plastoquinone deficiency but retained lamellar structure in a T-DNA mutant of Arabidopsis (2005)
  • Author(s): H.Shimada, et al.
  • Journal Title: The Plant Journal 41 Pages: 627-637
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Formation of Zn-Chl α in Chlorella heterotrophically grown in the dark with an excessive amount of Zn^<2+> (2005)
  • Author(s): I.Ikegami, et al.
  • Journal Title: Plant Cell Physiol. 46 Pages: 729-735
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Energy trans fer and charge separation dynamics in Chlorophyll d-based photosystem I from Acaryochloris marina (2005)
  • Author(s): Kumazaki, S., Ikegami, I., Itoh, S.
  • Journal Title: Photosynthesis : Fundamental Aspects to Global Perspectives (eds.A.van der Est, D.Bruce) Pages: 92-94
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The energy gap between P700^+-A_0^- and P700^* in photosystem I determined by delayed fluorescence at 270-77K (2005)
  • Author(s): Shibata, Y., Kasahara, T., Akai, S., Itoh, S., Ikegami, I.
  • Journal Title: Photosynthesis : Fundamental Aspects to Global Perspectives (eds.A.van der Est, D.Bruce) Pages: 94-97
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Zn-Chl a is accumulated functionally in Chlorella hetero-trophycally grown in the dark with an excessive amount of Zn^<2+> (2005)
  • Author(s): Ikegami, I., Nemoto, A., Sakashita, K.
  • Journal Title: Photosynthesis : Fundamental Aspects to Global Perspectives (eds.A.van der Est, D.Bruce) Pages: 157-158
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Inactivation and deficiency of core proteins of photosystems I and II caused by genetical phylloquinone and plastoquinone deficiency but retained lamellar structure in a T-DNA mutant of Arabidopsis (2005)
  • Author(s): H.Shimada, R.Ohno, M.Shibata, I.Ikegami, K.Onai, M.Ohta, K.Takamiya
  • Journal Title: The Plant Journal 41 Pages: 627-637
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Formation of Zn-Chla in Chlorella heterotrophically grown in the dark with an excessive amount of Zn^<2+> (2005)
  • Author(s): I.Ikegami, A.Nemoto, K.Sakashita.
  • Journal Title: Plant Cell Physiol 46 Pages: 729-735
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Energy equilibration among the electron carrier Chlorophylls in Photosystem I Reaction Center from Spinach (2003)
  • Author(s): Kumazaki, S., et al.
  • Journal Title: J. Phys. Chem. A, 107 Pages: 3228-3235
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Energy equilibration among the electron carrier Chlorophylls in Photosystem I Reaction Center from Spinach (2003)
  • Author(s): Kumazaki, S., Ikegami, I., Furusawa, H., Yoshihara, K.
  • Journal Title: J.Phys.Chem.A 107 Pages: 3228-3235
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] 光合成事典 (2003)
  • Author(s): 日本光合成研究会編
  • Total Pages: 430
  • Publisher: 学会出版センター
  • Description: 「研究成果報告書概要(和文)」より