2004 Fiscal Year Final Research Report Summary
Genomic analysis of MHC class I and MIC gene family region in primates
| Project/Area Number |
15570199
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anthropology
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| Research Institution | RIKEN |
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Principal Investigator |
FUKAMI Kaoru RIKEN, Bioresource Information Division, Senior Scientist, 情報解析技術室, 室長(副主任研究員待遇) (20225494)
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| Co-Investigator(Kenkyū-buntansha) |
INOKO Hidetoshi Tokai University School of Medicine, Division of Molecular Life Science, Professor, 医学部, 教授 (10101932)
TATENO Yoshio National Institute of Genetics, Center for Information Biology and DNA Data Bank of Japan, Professor, 国立遺伝学研究所・生命情報・DDBJ研究センター, 教授 (00202424)
IWASE Shigeru RIKEN, Bioresource Information Division, Research Scientist, 情報解析技術室, 研究員 (10391872)
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| Project Period (FY) |
2003 – 2004
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| Keywords | primate / MHC / MIC / genome / immunology / molecular evolution / chimpanzee / rhesus monkey |
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| Research Abstract |
To elucidate the origins of the MHC-B-MHC-C pair and the MICA-MICE pair, we sequenced a MHC class I genomic region of man, chimpanzee and Rhesus monkey, and analyzed them from an evolutionary standpoint, focusing first on LINE sequences that are paralogous within each of the first two species and orthologous between them. Since the LINE sequences turned out to be all fragmented and nonfunctional, they were suitable for conducting phylogenetic study and, in particular, for estimating evolutionary time.
Our study has revealed that MHC-B and MHC-C duplicated 22.3 million years(Myr) ago, and so did the ape MICA and MICE 14.1 Myr ago. We then estimated the divergence time of Rhesus monkey using other orthologous LINE sequences in the class I regions of the three primate species. The result indicates that Rhesus monkey, and possibly the old world monkey in general, diverged 27-30 Myr ago.
Interestingly, Rhesus monkey was found to have not the pair of MHC-B and MHC-C but many repeated genes similar to MHC-B. These results support our inference that MHC-B and MHC-C duplicated after the divergence between apes and old world monkeys.
The results of this research project were submitted to Proceedings of the National Academy of Science of the USA and have recently been accepted.
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