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2004 Fiscal Year Final Research Report Summary

Identification of genes related to expression of hybrid lethality in the cells of interspecific hybrid tobacco.

Research Project

Project/Area Number 15580003
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionIbaraki University

Principal Investigator

MARUBASHI Wataru  Ibaraki University, School of Agriculture, Professor, 農学部, 教授 (00181826)

Project Period (FY) 2003 – 2004
Keywordstobacco / hybrid lethality / apoptosis / programmed cell death
Research Abstract

In the cross Nicotiana suaveolens x N.tabacum, Yamada et al. (2001) reported that, using suspension cultured cells, hybrid lethality is expressed at 28℃, but suppressed at 36℃ and cell death during hybrid lethality in this hybrid is apoptotic and performed under genetic control. Masuda et al. (2003) reported that the point of no return, when the factor(s) controlling hybrid lethality are expressed, is 3 h after exposure to 28℃ and after the point of no return de novo transcription and translation are not required. In the present study, we attempted to isolate the genes expressing during hybrid lethality using the cDNA subtraction method. Poly(A)+RNA was isolated from a lethal hybrid line (LH line) and a surviving hybrid line (SH line) exposed to 28℃ for 5 h. Subtractive hybridization was performed using a PCR-select cDNA subtraction kit (Clontech) following the manufacturer's instructions. The subtracted cDNAs were cloned into a pGEM-T-easy vector (Promega) and secondary screening was performed using a PCR-select differential screening kit (Clontech). Eighteen positive clones were obtained and sequenced. A search using the BLASTX program identified similar sequences deposited within the GenBank database. To investigate the expression of genes specific to hybrid lethality, the real-time quantitative PCR was performed using an iQ SYBR green super mix (BioRad) on cDNA Obtained from the LH and SH lines exposed to 28℃ for 0,3,and 6 h. As a result, senescence (LH16,LH84), hypersensitive response (LH25), and auxin-induced protein (LH3)-related genes were expressed specifically in the LH line. This data suggests that hybrid lethality may possess similar mechanisms in the commitment or execution phases with senescence or hypersensitive response.

  • Research Products

    (12 results)

All 2006 2005 2004 2003

All Journal Article (12 results)

  • [Journal Article] Hybrid lethality in interspecific hybrids between Nicotiana tabacum and N.suaveolens : evidence that the Q chromosome causes hybrid lethality based on Q-chromosome-specific DNA markers.2006

    • Author(s)
      T.Tezuka, W.Marubashi
    • Journal Title

      Theoretical and Applied Genetics 112(In press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Hybrid lethality in interspecific hybrids between Nicotiana tabacum and N.suaveolens : evidence that the Q chromosome causes hybrid lethality based on Q-chromosome-specific DNA markers.2006

    • Author(s)
      T.Tezuka, W.Marubashi
    • Journal Title

      Theoretical and Applied Genetics 112(in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Heat treatment temporarily suppresses expression of programmed cell death in hybrid tobacco cells (Nicotiana suaveolens x N.tabacum) expressing hybrid lethality.2005

    • Author(s)
      S.Kobori, Y.Masuda, W.Marubashi
    • Journal Title

      Plant Biotechnology 22

      Pages: 345-348

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Apoptotic cell death observed during expression of hybrid lethality in interspecific hybrids between Nicotiana tabacum and N.suaveolens.2004

    • Author(s)
      T.Tezuka, W.Marubashi
    • Journal Title

      Breeding Science 54

      Pages: 59-66

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Temperature-dependent programmed cell death detected in hybrids between Nicotiana langsdorffii and N.tabacum expressing lethality.2004

    • Author(s)
      H.Watanabe, W.Marubashi
    • Journal Title

      Plant Biotechnology 21

      Pages: 151-154

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Programmed cell death detected in interspecific hybrids of Nicotiana repanda x N. tomentosiformis expressing hybrid lethality.2004

    • Author(s)
      S.Kobori, W.Marubashi
    • Journal Title

      Breeding Science 54

      Pages: 347-350

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Development of Q chromosome-specific DNA markers in tobacco and their use for identification of a tobacco monosomic line.2004

    • Author(s)
      T.Tezuka, K.Onosato, S.Hijishita, W.Marubashi
    • Journal Title

      Plant and Cell Physiology 45

      Pages: 151-154

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Programmed cell death detected in interspecific hybrids of Nicotiana repanda x N.tomentosiformis expressing hybrid lethality.2004

    • Author(s)
      S.Kobori, W.Marubashi
    • Journal Title

      Breeding Science 54

      Pages: 347-350

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Time course analysis of apoptosis during hybrid lethality in tobacco hybrid cells (Nicotiana suaveolens x N. tabacum).2003

    • Author(s)
      Y.Masuda, T.Yamada, W.Marubashi
    • Journal Title

      Plant and Cell Physiology 44

      Pages: 420-427

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Over-produced ethylene causes apoptotic cell death leading to temperature-sensitive lethality in hybrid seedlings from the cross of Nicotiana suavelns x N.tabacutm.2003

    • Author(s)
      T.Yamada, W.Marubashi
    • Journal Title

      Planta 217

      Pages: 690-698

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Time course analysis of apoptosis during hybrid lethality in tobacco hybrid cells (Nicotiana suaveolens x N.tabacum).2003

    • Author(s)
      Y.Masuda, T.Yamada, W.Marubashi
    • Journal Title

      Plant and Cell Physiology 44

      Pages: 420-427

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Over-produced ethylene causes apoptotic cell death leading to temperature-sensitive lethality in hybrid seedlings from the cross of Nicotiana suavelens x N.tabacum.2003

    • Author(s)
      T.Yamada, W.Marubashi
    • Journal Title

      Planta 217

      Pages: 690-698

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2007-12-13  

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