2005 Fiscal Year Final Research Report Summary
Characterization of humic substances aimed at using for the removal of pollutants
| Project/Area Number |
15580045
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | Hirosaki University |
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Principal Investigator |
AOYAMA Masakazu Hirosaki University, Faculty of Agriculture and Life Science, Proffesor, 農学生命科学部, 教授 (60150950)
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| Project Period (FY) |
2003 – 2005
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| Keywords | himic substances / environmental pollution / RP-HPLC / heavy metals / PAHs |
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| Research Abstract |
The hydrophilic property of humic substances is related to the complexation with heavy metals, whereas the hydrophobic property with the adsorption of polycyclic aromatic hydrocarbons (PAHs). To characterize humic substances based on their hydrophilicity and hydrophobicity, reverse-phased high performance liquid chromatography (RP-HPLC) was applied to humic and fulvic acids. The fractions responsible for the complexation with heavy metals and for the adsorption of PAHs were estimated by RP-HPLC with fluorescence detection. Furthermore, the effects of humic substances on the absorption of heavy metals by plant were investigated.
1.When pH gradient elution and acetonitrile concentration gradient elution were applied to RP-HPLC using a C18 polymer column, humic acids were separated into three fractions according to their hydorphilicity and hydrophobicity.
2.When used a polymer monolith column was used for RP-HPLC to increase resolving power, humic and fulvic acids were separated into five f
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ractions (I-V). The fraction I was most hydrophilic, whereas the fraction V most hydrophobic. Fulvic acids were characterized by a dominance of fraction I. For humic acids, however, the composition varied with the degree of humification. The principal component analysis using the peak area of each fraction showed that humic acid and fulvic acid were discriminated by the peak area of fraction V and the A type humic acids were discriminated from the other types of humic acids based on the peak areas of fractions II-IV.
3.It was estimated from the quenching of fluorescence that the fractions responsible for the complexation with heavy metals were the fractions I and II for humic acids and only the fraction I for fulvic acids. On the other hand, the fractions responsible for the adsorption of PAHs were considered to be the all the fractions for humic acids and the fractions II to IV for fulvic acids based on the increase in the fluorescence when pyrene was react with the humic substances.
4.The application of compost-derived humic substances increased the solubility of Cu in soil, and thus increased the absorption of Cu by Indian mustard (Brassica juncea). Less
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[Journal Article] The role and function of soil organic constituents in the terrestrial ecosystem and environment.2004
Author(s)
Tsutsuki, K., Sumida, H., Aoyama, M., Shindo, H., Miyajima, T., Kawahigashi, M., Fujitake, N.
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Journal Title
Japanese Journal of Soil Science and Plant Nutritio 75-4
Pages: 511-517
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Humus.2004
Author(s)
Aoyama, M.
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Journal Title
The Compendium of Agricultural Technology. Soil fertilizers (Nohbunkyo Publishers) Vol.4, No.15
Description
「研究成果報告書概要(欧文)」より
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