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2004 Fiscal Year Final Research Report Summary

Establishment of methods for prediction of the function of functionally-unknown gene products by the analysis of the posttranslational protein modifications.

Research Project

Project/Area Number 15580080
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied biochemistry
Research InstitutionYamaguchi University

Principal Investigator

UTSUMI Toshihiko  Yamaguchi University, Department of Biological Chemistry, Faculty of Agriculture, Professor, 農学部, 教授 (20168727)

Project Period (FY) 2003 – 2004
Keywordsprediction / protein function / functionally-unknown protein / posttranslational modification / protein N-myristoylation / modification signal
Research Abstract

Upon the completion of the Human Genome Project, huge numbers of genetic sequences are now available. However, the function of many of these genes has not been identified. Therefore, the identification of the function of these genes is one of the most important purposes of the study of Proteomics.
Now, it is well known that many of the posttranslational modifications such as proteolytic processing, phosphorylation, glycosylation, lipid modifications, ADP-ribosylation etc. play critical roles in the structure and function of protein and regulate many cellular events such as intracellular signal transduction. However, systematic method to analyze the posttranslational modification of protein has not been established.
In the present study, we have shown that targeting, processing and modification of a distinct protein could be successfully detected by using metabolic labeling in an in vitro translation system or in transfected cells. Since many of these posttranslational modifications play specific roles in the structure and function of protein, it might be possible to predict the function of the protein from these experimental observations.

  • Research Products

    (15 results)

All 2005 2004 2003 Other

All Journal Article (14 results) Book (1 results)

  • [Journal Article] N-terminus of B96Bom, a Bombyx mori G-protein-coupled receptor, is N-myristoylated and translocated across the membrane.2005

    • Author(s)
      Utsumi, T. et al.
    • Journal Title

      FEBS Journal 272

      Pages: 472-481

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] N-terminus of B96Bom, a Bombyx mori G-protein-coupled receptor, is N-myristoylated and translocated across the membrane.2005

    • Author(s)
      Utsumi, T., Ohta, H., Kayano, Y., Sakural, N., Ozoe, Y.
    • Journal Title

      FEBS Journal 272

      Pages: 472-481

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Vertical-scanning mutagenesis of amino acids in a model N-myristoylation motif reveals the major amino-terminal sequence requirements for protein N-myristoylation2004

    • Author(s)
      Utsumi, T. et al.
    • Journal Title

      European Journal of Biochemistry 271

      Pages: 863-874

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] N-ミリストイル化によるタンパク質の膜局在化と機能調節2004

    • Author(s)
      内海俊彦
    • Journal Title

      実験医学 22

      Pages: 1252-1259

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Oxidative stress underlies the mechanism for Ca^<2+>- induced permeability transition of mitochondria.2004

    • Author(s)
      Kanno, T., Sato, E.F., Muranaka, S., Fujita, H., Utsumi, T., Inoue, M., Utsumi, K.
    • Journal Title

      Free Rad.Res. 38

      Pages: 27-35

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Vertical-scanning mutagenesis of amino acids in a model N-myristoylation motif reveals the major amino-terminal sequence requirements for protein N-myristoylation.2004

    • Author(s)
      Utsumi, T., Nakano, K., Funakoshi, T., Kayano, Y., Nakao, S., Sakurai, N., Iwata, H., Ishisaka, R.
    • Journal Title

      Eur.J.Biochem. 271

      Pages: 863-874

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Identification of amino acid residues interacting with tyramine in the Bombyx mori tyramine receptor.2004

    • Author(s)
      Ohta, H., Utsumi, T., Ozoe, Y.
    • Journal Title

      Insect Molecular Biology 13

      Pages: 531-538

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] B96Bom encodes a Bomyx mori tyramine receptor negatively coupled to adenylate cyclase2003

    • Author(s)
      Ohta, H. et al.
    • Journal Title

      Insect Molecular Biology 12

      Pages: 217-223

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Topogenesis of two transmembrane type K^+ channels, Kir2.1 and KcsA2003

    • Author(s)
      Umigai, N. et al.
    • Journal Title

      Journal of Biological Chemistry 278

      Pages: 40373-40384

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] In vitro及びin vivo代謝標識法を用いた蛋白質翻訳後修飾の解析2003

    • Author(s)
      内海俊彦
    • Journal Title

      生化学 75

      Pages: 373-378

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] B96Bom encodes a Bomyx mori tyramine receptor negatively coupled to adenylate cyclase.2003

    • Author(s)
      Ohta, H., Utsumi, T., Ozoe, Y.
    • Journal Title

      Insect Molecular Biology 12

      Pages: 217-223

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Mechanism of enhanced apoptosis in HL-60 cells by UV-irradiated n-3 and n-6 polyunsaturated fatty acids.2003

    • Author(s)
      Arita, K., Yamamoto, Y., Takehara, Y., Utsumi, T., Kanno, T., Miyaguchi, C., Akiyama, J., Yoshioka, T., Utsumi, K.
    • Journal Title

      Free Radic.Biol.Med. 35

      Pages: 189-199

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Topogenesis of two transmembrane type K^+ channels, Kir2.1 and KcsA.2003

    • Author(s)
      Umigai, N., Sato, Y., Mizutani, A., Utsumi, T., Sakaguchi, M., Uozumi, N.
    • Journal Title

      J.Biol.Chem. 278

      Pages: 40373-40384

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Involvement of Ras/Extracellular signal-regulated kinase, but not Akt pathway in risedronate-induced apoptosis of U937 cells and its suppression by cytochalasin B.

    • Author(s)
      Fujita, H., Utsumi, T., Muranaka, S., Ogino, T., Yano, H., Akiyama, J., Yasuda, T., Utsumi, K.
    • Journal Title

      Biochem Pharmacol. (In press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] 「膜局在性のタンパク質工学的改変による生理活性タンパク質の機能変換」タンパク質工学2003

    • Author(s)
      内海俊彦 ら
    • Total Pages
      165-204
    • Publisher
      医学書院
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2006-07-11  

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