2005 Fiscal Year Final Research Report Summary
Bioactive natural compounds obtained from arthropods
| Project/Area Number |
15580090
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
MORI Naoki Kyoto University, Graduate School of Agriculture, Assistant Professor, 農学研究科, 助教授 (30293913)
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| Project Period (FY) |
2003 – 2005
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| Keywords | volicitin / elicitor / induced resistance / pumiliotoxins / Dendrobatid alkaloids / atopic dermatitis / α-acaridial / house-dust mites |
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| Research Abstract |
1) Volicitin [N-(17-hydroxylinolenoyl)-L-glutamine] is known as an insect-produced plant volatiles elicitor. In this study, the in vitro biosynthesis of volicitin was demonstrated for the first time by using gut tissues of Spodoptera litura larvae. The absolute configuration of the hydroxylinolenoyl moiety of volicitin was determined to 17-S in high enantiomeric excess in S.litura. Furthermore, N-linolenoyl-L-glutamine was only about 30% as active as volicitin. Among synthesized volicitin-related compounds, only glutamine conjugate induced the emission of volatile compounds from corn seedlings. On the other hand, we found uktraweak phton emissions from maize leaves damaged by Heilcovera armigera. Interestingly, the direct interaction between maize leaf cells and chemicals obtained from caterpillar regurgitants triggers the phton emissions.
2) Pumiliotoxins are very widely distributed in alkaloids-containing anurans from the neotropics. We demonstrated pumiliotoxin 237A, 251D and 8-deoxypumiliotoxin 193H from oribated mite extracts. It appears possible that some of the pumiliotoxins and related congeners found in extracts of various anurans have amite origin
3) Some particular substances obtained from mites, such as secretions from opisthonothal glands are suspected as one of antigens. We happened to find α-acaridial showed a positive reaction in patch tests for atopic dermatitis patients. In this study, we demonstrated that the female sex pheromone of Rhizoglyphus setosus (Astigmata : Acaridae) was identified as S-robinal, which is analogous to α-acaridial. Furthermore, we established the species-specific ITS sequence useful for the species identification of astigmatid mites and for studying low-level phylogenetic relationships.
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Research Products
(13 results)
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[Journal Article] Ultraweak phton emission from herbivory-injured maize plants.2006
Author(s)
Yoshinaga, N., Kato, K., Kageyama, C., Fujisaki, K., Nishida, R., Mori, N.
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Journal Title
Naturwissenschaften 93
Pages: 38-41
Description
「研究成果報告書概要(和文)」より
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[Journal Article] A hemiterpene glucoside as a probing deterrent of the bean aphid, Megoura crassicauda, from a non-host vetch, Vicia hirsute.2006
Author(s)
Ohta, N., Mori, N., Kuwahara, Y., Nishida, R.
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Journal Title
Phytochemistry 67
Pages: 584-588
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Ultraweak phton emission from herbivory-injured maize plants.2006
Author(s)
Yoshinaga, N., Kato, K., Kageyama, C., Fujisaki, K., Nishida, R., Mori, N.
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Journal Title
Naturwissen-schaften 93
Pages: 38-41
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Identification of astigmatid mites using the second internal transcribed spacer (ITS2) region and its application for phyrogenetic study.2005
Author(s)
Noge, K., Mori, N., Tanaka, C., Nishida, R., Tsuda, M., Kuwahara, Y.
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Journal Title
Experimental and Applied Acarology 35
Pages: 29-46
Description
「研究成果報告書概要(和文)」より
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[Journal Article] S-Isorobinal as the female sex pheromone from an alarm pheromone-emitting mite, Rizoglyphus setosus.2005
Author(s)
Mizoguchi, A., Murakami, K., Shimuzu, N., Mori, N., Nishida, R., Kuwahara, Y.
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Journal Title
Experimental and Applied Acarology 36
Pages: 2405-2417
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Scheloribatid mites as the source of pumiliotoxins in dendrobatid frogs.2005
Author(s)
Takada, W., Sakata, T., Shimano, S., Enami, Y., Mori, N., Nishida, R., Kuwahara, Y.
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Journal Title
Journal of Chemical Ecology 31
Pages: 2405-2417
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Biosynthesis of neral in Carpoglyphus lactis (Acari : Carpoglyphidae) and detection of its key enzyme, geraniol dehydrogenase, by electrophoresis.2005
Author(s)
Noge, K., Kato, M., Iguchi, T., Mori, N., Nishida, R., Kuwahara, Y.
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Journal Title
Journal of the Acalorogical Society of Japan 14
Pages: 75-81
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Absolute configuration of volicitin from regurgitant of lepidopteran caterpillars and biological activity of volicitin-related compounds.
Author(s)
Sawada, Y., Yoshinaga, N., Fujisaki, K., Nishida, R., Kuwahara, Y., Mori, N.
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Journal Title
Bioscience, Biotechnology and Biochimistry (accepted)
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Absolute configuration of volicitin from regurgitant of lepidopteran caterpillars and biological activity of volicitin-related compounds.
Author(s)
Sawada, Y., Yoshinaga, N., Fujisaki, K., Nishida, R., Kuwahara, Y., Mori, N.
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Journal Title
Bioscience, Biotechnology and Biochimistry (accepted)
Description
「研究成果報告書概要(欧文)」より
