2004 Fiscal Year Final Research Report Summary
Cryopreservation of dolphin spermatozoa
| Project/Area Number |
15580163
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | Mie University |
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Principal Investigator |
YOSHIOKA Motoi Mie University, Faculty of Bioresources, Associate professor, 生物資源学部, 助教授 (30262992)
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| Project Period (FY) |
2003 – 2004
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| Keywords | dolphin / sperm / cryopreservation / artificial insemination / straw method / cetacean / marine mammals / reproductive physiology |
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| Research Abstract |
In order to develop an effective and simple method for cryopreserving dolphin semen processed immediately after collection by the hand-manipulation method, we conducted some freezing and thawing experiments by straw method for fresh semen samples obtained from two bottlenose dolphins (Tursiops truncatus) and one Pacific white-sided dolphins (Lagenorhynchus obliquidens) in captivity. For both dolphin species, post-thaw sperm with total motility about 60% or more, percentage progressive motility over 95% and kinetic rating 4 to 5 (rapid forward progressive movement) were obtained by the straw method, with semen extender containing 2.9% trisodium citrate hydrate, 8% glycerol, 20% egg-yolk and antibiotics. Fresh semen was diluted twice with the semen extender to the final dilution 1:2 (semen : extender, v/v) and precryopreserved at 4 degrees C (refrigerator;1-1.5 hr) at the first dilution and then at 2 degree C (ice-water bath;0.25-1hr) at the second dilution. About 300-400 micro litters of extended semen put into 0.5mL-straw were frozen in the liquid nitrogen vapor for 15 min. and then straws were plunged into liquid nitrogen for complete freezing. Rapid thawing in the water bath at 35-36 degrees C led into better post-thaw semen characteristics. Post-thaw sperm frozen by the present straw method was used for the artificial insemination(AI) to a female bottlenose dolphin in September 2003,and a male calf was born as the second AI dolphin baby (the first with frozen-thawed sperm) in Japan in September 2004,
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Research Products
(4 results)
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[Journal Article] Estrous cycle characterization and artificial insemination using frozen-thawed spermatozoa in the bottlenose dolphin (Tursiops truncatus).2005
Author(s)
Robeck T.R., Steinman, K.J., Yoshioka, M., Jensen, E., O'Brien, J.K., Katsumata, E., Gili, C., McBain, J.F., Sweeney, J., Monfort, S.L.
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Journal Title
Reproductin 129
Pages: 659-674
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Artificial insemination using frozen-thawed semen in the Pacific white-sided dolphin (Lagenorhynchus obliquidens).2003
Author(s)
Robeck.T.R., Greenwell, M., Boehm, J.R., Yoshioka, M., Tobayama, T., Steinman, K., Monfort, S.L.
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Journal Title
Proceedings for the 34th Annual Conference of the International Association for Aquatice Animal Medicine, May 9-14,2003, Waikoloa, Hawaii
Pages: 50-52
Description
「研究成果報告書概要(欧文)」より
