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2004 Fiscal Year Final Research Report Summary

Induction of mucin gene expression by lactic acid bacteria adhering to intestinal epithelial cells

Research Project

Project/Area Number 15580237
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Zootechnical science/Grassland science
Research InstitutionKitasato University

Principal Investigator

MUKAI Takao  Kitasato University, School of Veterinary Medicine and Animal Sciences, Associate Professor, 獣医畜産学部, 助教授 (20229917)

Project Period (FY) 2003 – 2004
Keywordslactic acid bacteria / mucin / induction / adhesion / Caco-2
Research Abstract

Probiotics are microbial cell preparations or components of microbial cells that have a beneficial effect on the health and well-being of the host. Selected probiotics have been shown to have significant health benefits for humans, and thus several well-characterized strains are available for human use to reduce the risk of gastrointestinal infections or to treat such infections. Some Lactic acid bacteria including lactobacilli and bifidobacteria have been used as probiotics. The aim of this project is to examine whether adhesive lactobacilli and bifidobacteria have an ability to induce expression of innate immunity-relating genes such as mucin and several cytokine genes in intestinal epithelial cells. For the purpose, Caco-2 as well as HT-29 and Intestine-407 were used as human colonic cell lines.
Several strains of Lactobacillus reuteri, Lactobacillus acidophilus group, Bifidobacterium longum and Bifidobacterium bifidum were found to adhere to the human colonic cell lines. Some of adhesive strains were found to induce weakly mucin gene expression, while some strains strongly induced the anti-bacterial peptide gene including β-defensin and several cytokine genes. The gene expression seems to be dependent on the adhesion ability of the bacteria tested. It was also found that the gene response against bacterial cells was different from cell lines, i.e. HT-29 cells were adequate to examine the expression of mucin gene while Caco-2 cells were adequate to examine the expression of the anti-bacterial peptide gene and cytokine genes. The strains having the ability to induce mucin and anti-bacterial peptide genes can be considered for future in vivo challenge studies in human as probiotics.

  • Research Products

    (4 results)

All 2004 2003

All Journal Article (4 results)

  • [Journal Article] Binding of Bifidbacteriun bifidum and Lactobacillus reuteri to the carbohydrate moieties of intestinal glycolipids recognized by peanut agglutinim.2004

    • Author(s)
      Mukai T et al.
    • Journal Title

      Int.J.Food Microbiol. 90

      Pages: 357-362

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Cell surface-associated proteinaceous substances of Bifidobacterium bifidum are involved in binding to the carbohydrate moieties of intestinal glycolipids recognized by peanut agglutinin.2004

    • Author(s)
      Mukai, T., Kaneko, S., Hashizume, W., Ohori, H.
    • Journal Title

      Int.J.Food Microbiol. 90

      Pages: 357-362

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Molecular mechanism of adhesion of lactobacilli to mucosal surface.2003

    • Author(s)
      Mukai, T., Toba, T., Ohori, H.
    • Journal Title

      Milk Science(in Japanese) 52,3

      Pages: 167-174

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Lactobacillus kitasatonis sp.nov., from chicken intestine.2003

    • Author(s)
      Mukai, T., Arihara, K., Ikeada, A., Nomura, K., Suzuki, F., Ohori, H.
    • Journal Title

      Int.J.Syst.Evol.Microbiol. 53

      Pages: 2055-2059

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2006-07-11  

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