2004 Fiscal Year Final Research Report Summary
Control of the synthesis of nitrite reductase in ruminal bacteria.
| Project/Area Number |
15580240
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Zootechnical science/Grassland science
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| Research Institution | Meiji University |
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Principal Investigator |
HINO Tsuneo Meiji University, School of Agriculture, Professor, 農学部, 教授 (50012050)
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| Project Period (FY) |
2003 – 2004
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| Keywords | Ruminal microbes / Nitrate / nitrite reduction / Nitrate reductase / Selenomonas ruminantium / Wolinella succinogenes / Streptococcus bovis / Methane suppression / Global catabolite control |
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| Research Abstract |
1.Nitrate reductase of Selenomonas ruminantium was purified, and its gene was sequenced. Factors affecting the transcription of the gene were examined.
2.The cell number of S.ruminantium having the capacity to reduce nitrate and nitrite in the rumen was found to be about a 10 % of the total number of S.ruminantium. Therefore, it is desirable to stimulate the growth of nitrate- and nitrite-reducing strains. Augmentation of fiber digestion was suggested to suppress nitrite accumulation in the rumen.
3.A species of Clostridium that has high capacity to reduce nitrite was isolated. Addition of the bacterium to the ruminal microbiota in vitro resulted in an increase in the rate of nitrite reduction and a decrease in methane production.
4.Similarly, addition of Wollinella succinogenes that has high capacity to reduce nitrite increased the rate of nitrite reduction and decreased methane production. Further supplementation of nitrate and fumarate magnified the effects.
5.Addition of ethanol to the ruminal microbiota stimulated nitrate and nitrite reduction.
6.A strain of Streptococcus bovis that has high capacity to reduce nitrite was isolated. As mechanisms to control fermentation and growth in S.bovis, importance of the involvement of global catabolite control protein A, fructose-1,6-bisphosphate, and LuxS autoinducer 2 was shown.
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