2004 Fiscal Year Final Research Report Summary
The roles of endogenous retroviruses as cofactors of pathogenic retroviral infections
| Project/Area Number |
15580258
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Obihiro University of Agriculture and Veterinary Medicine (2004)
Osaka University (2003) |
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Principal Investigator |
MIYAZAWA Takayuki Obihiro University of Agriculture and Veterinary Medicine, Department of Applied Veterinary Medicine, Associate Professor, 畜産学部, 助教授 (80282705)
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| Co-Investigator(Kenkyū-buntansha) |
OHNO Koh-ichi The University of Tokyo, Department of Veterinary Internal Medicine, Associate Professor, 大学院・農学生命科学研究科, 助教授 (90294660)
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| Project Period (FY) |
2003 – 2004
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| Keywords | FeLV / endogenous retroviruses / FeLIX / Receptors / Pit1 / AIDS / Cats / T lymphocytes |
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| Research Abstract |
T-lymphotropic feline leukemia virus (FeLV-T) is a highly pathogenic variants of FeLVs, and induces feline AIDS (FAIDS) in cats. FeLV-T is fusion- defective because its PHQ motif, gammaretroviral consensus motif in the N-terminal regions of envelope protein, has substitution of histidine to aspartate. Infection of FeLV-T requires FeLIX, a truncated envelope protein encoded by an endogenous FeLV (enFeLV) for transactivation its infectivity and Pit1 for FeLIX-binding. Although Pit1 distributes in many cells, the expression of FeLIX is expressed only in some lymphoid organs. Therefore, it has been thought that the host cell range of FeLV-T is restricted to cells expressing FeLIX. However, because FeLIX is a soluble factor and expressed constantly, we presumed that FeLIX is present in blood and evaluated FeLIX activities in sera of various mammalian species by LacZ pseudotype assay. Here, we demonstrated that cat serum has FeLIX activity at functional level, suggesting that FeLIX is present abundantly in the internal environment of a cat and FeLV-T may be able to infect cells expressing Pit1 regardless FeLIX expression in vivo. We also confirmed that FeLIX did not inhibit FeLV-B infection despite FeLIX and FeLV-B exhibit the same receptor usage. These results implies that FeLIX may work just negatively for the host defence against the infection of exogenous viruses.
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[Journal Article] The Effect of Expression of Complement Regulatory Protein on Pig Endothelial Cells is Pig Endogenous Retrovirus (PERV) lyses by human sera.2005
Author(s)
Hazama, K., Miyagawa, S., Yamamoto, A., Kubo, T., Miyazawa, T., Tomonaga, K., Watanabe, R., Okumura, M., Matsuda, H., Shirakura, R.
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Journal Title
Transplant Proc. 37
Pages: 503-505
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Prevention of PERV infections in pig to human xenotransplantation by the RNA interference silences gene.2005
Author(s)
Miyagawa, S., Nakatsu, S., Nakagawa, T., Kondo, A., Matsunami, K., Hazama, K., Yamda, J., Tomonaga, K., Miyazawa, T., Shirakura, R.
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Journal Title
J.Biochem. 137
Pages: 503-508
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Use of CD134 as a primary receptor by the feline immunodeficiency virus.2004
Author(s)
Shimojima, M., Miyazawa, T., Ikeda, YU., McMonagle, E.L., Haining, H., Akashi, H., Takeuchi, Y., Hosie, M.J., Willett, B.J.
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Journal Title
Science 303
Pages: 1192-1195
Description
「研究成果報告書概要(欧文)」より
