2005 Fiscal Year Final Research Report Summary
Mechanisms of differentiation of mesenchymal stem cells and their functional roles in diseases
| Project/Area Number |
15580262
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
KOTANI Takao Osaka Prefecture University, Life and Environmental Sciences, Professor, 生命環境科学研究科, 教授 (00081589)
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| Co-Investigator(Kenkyū-buntansha) |
YAMATE Jyoji Osaka Prefecture University, Life and Environmental Sciences, Associate Professor, 生命環境科学研究科, 助教授 (50150115)
SUGIURA Kikuya Osaka Prefecture University, Life and Environmental Sciences, Associate Professor, 生命環境科学研究科, 助教授 (30171143)
KUWAMURA Mitsuru Osaka Prefecture University, Life and Environmental Sciences, Assistant Professor, 生命環境科学研究科, 講師 (20244668)
KUMAGAI Daijirou Osaka Prefecture University, Life and Environmental Sciences, Research Assistant, 生命環境科学研究科, 助手 (70316016)
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| Project Period (FY) |
2003 – 2005
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| Keywords | Mesenchymal differentiation / Undifferentiated stem cell / Malignant fibrous histiocytoma / Malignant teratoma / Osteoblastic differentiation / Lipoblastic differentiation / Myofibroblastic differentiation / Rat tumor model |
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| Research Abstract |
To clarify the differential mechanisms of mesenchymal cells, the following experiments were performed using undifferentiated mesenchymal stem cell lines (MT-9, TSD-B9). MT-9 is a cell line isolated from a rat malignant fibrous histiocytoma having pluripotential differentiation, and TSD-B9 is a cell line derived from a rat malignant teratoma, of which the origin is gonadal germ cells capable of differentiating towards endodermal, mesodermal and ectodermal tissues.
1.Osteoblastic differentiation : The addition of a supplement for osteoblastic differentiation, including BMP-2, to these cell lines increased calcified areas, demonstrable by von Kossa stain, and showed an elevated alkaline phosphatase in supernatants ; these are indicators of osteoblastic differentiation.
2.Lipoblastic differentiation : The addition of a supplement for lipoblastic differentiation, including indomethacin, dexamethasone and insulin, to these cell lines increased the number of cells having oil red O-stainable lipids in the cytoplasm. The stain is useful for detection of lipoblastic cells.
3.Myofibroblastic differentiation : The treatment with TGF-β1, a fibrogenic factor in fibrotic diseases, increased the number of cells immuno-reacting to α-smooth muscle actin (α-SMA) ; the increase was demonstrated by the western blotting. α-SMA-expressing cells are regarded as myofibroblasts.
5.These studies partly clarified the mechanisms of mesenchymal differentiation, and these cell lines would prove useful for investigating the mechanisms.
6.Furthermore, a new cell line was established from a rat malignant fibrous histiocytoma arising in the subcutis, showing histiocytic nature, and another cell line was isolated from a rat malignant schwannoma generating from the uterine cervix. These cell lines would become also beneficial tools for studies on mesenchymal differentiation.
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