2005 Fiscal Year Final Research Report Summary
Investigation for the infectious cycle of rotaviruses in nature and prediction of the epidemics of novel rotaviruses
| Project/Area Number |
15580273
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | Gifu University |
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Principal Investigator |
SUGIYAMA Makoto Gifu University, United Graduate School of Veterinary Sciences, Professor, 連合獣医学研究科, 教授 (80196774)
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| Co-Investigator(Kenkyū-buntansha) |
MINAMOTO Nobuyuki Gifu University, Faculty of Applied Biological Sciences, Professor, 応用生物科学部, 教授 (10144007)
KITAGAWA Hitoshi Gifu University, Faculty of Applied Biological Sciences, Professor, 応用生物科学部, 教授 (70144003)
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| Project Period (FY) |
2003 – 2005
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| Keywords | Group A rotavirus / Seroepidemiological study / Purified VP8 expressed in E.coli / Latex agglutination test / P genotype / Zoonosis / Infectious cycle / Japanese encephalitis virus |
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| Research Abstract |
The latex agglutination (LA) tests for detection of P genotype-specific antibody to Group A rotavirus were established. The antibodies to P[2] and [3] rotaviruses, which were epidemiologically relevant in some animal species, were detected by the LA test using the purified VP8 of simian rotavirus SA-11 strain as a antigen (SA-LA test). Likewise, the antibodies to the most frequent P[4] and [8] rotaviruses in human and p[17] rotavirus in birds were detectable in the LA tests using the VP8s of human rotavirus Wa strain (Wa-LA test) and pigeon rotavirus PO-13 strain (PO-LA test), respectively. The seroepidemiological study on rotavirus infection in human, domestic and wild animals was performed by using the LA tests described above. The results in three tests suggested that these human and animals should intricately suffer the infection of various rotaviruses. Interestingly, the investigation by PO-LA test showed that avian p[17] rotavirus was prevalent in human, suggesting that avian rotavirus may be a zoonotic agent. From the results in three LA tests, it was indicated that P[2] or [3], [4] or [8], and [17] rotavirus infections would be highly frequent in cattle and wild boar and these animals might be infected with an unknown P genotype rotavirus. The results in this study provide the important information to prevent human and animals from the rotavirus infection. In the sera collected in this study, the epidemiological investigation on Japanese encephalitis virus was also performed by using neutralizing antibody test. This result suggests that wild boar may play a role as an amplifier in the infectious cycle of Japanese encephalitis viruses in Japan.
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