2004 Fiscal Year Final Research Report Summary
Development of Microbial Enzyme which separates Cadmium from Marine Wastes
| Project/Area Number |
15580304
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Boundary agriculture
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| Research Institution | Hakodate National College of Technology |
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Principal Investigator |
OBARA Toshiyuki Hakodate National College of Technology, Department of Civil Engineering, Professor, 環境都市工学科, 教授 (60177293)
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| Project Period (FY) |
2003 – 2004
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| Keywords | Marine Waste / Cadmium Separation / Microorganisms / Enzyme / Scallop Hepatopancreas / Squid Liver / Solubilization |
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| Research Abstract |
Dumping of scallop hepatopancreas and squid liver, a waste of scallop and squid processing, has become a serious problem in Hokkaido. These tissues contain a large amount of cadmium, which is a toxic heavy metal. Microbial removal of cadmium from a homogenate of hepatopancreas, was examined to use this waste for value-added protein resources. Hepatopancreas tissues were digested (solubilized) by the various market proteases. Yield of solubilization of the organ in one protease treatment was 83%. During cultivation of the strain Xanthomonas sp. UR No.2 in the homogenate of hepatopancreas digested by the proteases, 96.0% of cadmium was removed. That is, these enzyme and organism are effective for the removal of cadmium from the protease treated homogenate of hepatopancreas.
Microorganisms were screened on the basis of the ability to grown in the medium with scallop hepatopancreas or squid liver as a carbon and nitrogen sources by enrichment culture. Enrichment culture from soil and activated sludge yielded eight different bacteria. Soil bacteria isolated were assayed by protease activity of culture supernatant with model experiment. One soil isolate has high extracellular protease activity, resulting in an enhancement of solubilization of protein of scallop hepatopancreas and squid liver tissues.
Solubilization of testis (gonad), a waste of scallop processing, is required from the viewpoint of the preparation of value-added protein resources. Microorganisms were screened on the basis of the ability to grow in the medium with scallop testis as a carbon and nitrogen sources by enrichment culture. One soil isolate, has high extracellular protease activity, resulting in an enhancement of solubilization of scallop testis tissues.
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