2005 Fiscal Year Final Research Report Summary
A structure scientific study about interaction of polyamine and left-handed DNA.
| Project/Area Number |
15590051
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Osaka University of Pharmaceutical Sciences |
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Principal Investigator |
OHISHI Hirofumi Osaka University of Pharmaceutical Sciences, Associate Professor, 薬学部, 講師 (00211095)
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| Project Period (FY) |
2003 – 2005
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| Keywords | X-ray crystallography / Z-DNA / B-Z junction / B-Z transfer / minor groove / groove binder / B-Z combination crystal / minor groove cavity |
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| Research Abstract |
I understood that N1-(2-(2-aminoethylamino)ethyl)ethane-1,2-diamine PA (222) and N1-(2-(2-(2-aminoethylamino)ethylamino)ethyl)ethane-1,2-diamine PA (2222) were easy to be connected in a minor groove of Z-DNA very much and stabilized Z-DNA as a minor groove binder very much. Furthermore, I crystallize at 4 degrees Celsius, 10 degrees Celsius, 20 degrees Celsius by the high salt density with these 11 kinds of polyamine and d(CG)3 as a complex and give the salt density at 20 degrees Celsius very much and crystallize. I examine a role for Z-DNA of polyamine, stabilization action in detail by examining these. I perform crystallization of a long chain of Z-DNA which it has been said to that the above-mentioned crystallization vs. eight bases is difficult till now if I understand stabilization mechanism. I think to clarify structure of DNA such as mixture type d(CGCGCGCGCGAATTCGCGCGCGCG)2, d(CGCGCGCGAATTCGCGCGCG)2 and d(CGCGCGAATTCGCGCG)2 of B type DNA and Z type DNA to clarify B-Z transfer m
… More
echanism, Z-B transfer mechanism. As for the structure of DNA of B-Z mixture type, crystallization is tried all over the world till now, but there is not a report besides an example of our crystallization. I think about letting we bury a cavity of the big minor groove that it is it in an element of destabilization with this polyamine with the polyamine which is a minor groove binder as DNA and a complex of these long chains and stabilize structure of the whole DNA. Therefore we succeed in X-ray crystallographic analysis of left-handed DNA of a long chain. As for the structure of DNA of B-Z mixture type, crystallization is tried all over the world till now, but there is not a report besides an example of our crystallization. The groove binder polyamine which it is crawled, and I discovered is connected in a minor groove of just Z-DNA and I bury a big minor groove and stabilize structure of the whole DNA. I apply this and perform crystallization of DNA of a long chain, crystallization of B-Z-B junction structure. I think that I bring big development in structured study of DNA if I have already succeeded in crystallization of the DNA which is the longest in the world with this method now, and structure or B-Z-B junction structure of DNA of a long chain are clarified in sequence Less
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[Journal Article] Intermolecular association model of antifungal polyene macrolide via concerted =C-H… O interactions : X-ray crystal structure analysis and ab initio molecular-orbital calculations of chainin.2006
Author(s)
In, Yasuko, Ohishi, Hirofumi, Miyagawa, Hiroo, Kitamura, Kunihiro, Igarashi, Yasuhiro, Ishida, Toshimasa.
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Journal Title
Bulletin of the Chemical Society of Japan 79
Pages: 126-133
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Synthesis and properties of 2'-0,4'-C-methylene-oxy-methylene bridged nucleic acid.2006
Author(s)
Hari, Yoshiyuki, Obika, Satoshi, Ohnishi, Ryo, Eguchi, Ken, Osaki, Tomohisa, Ohishi, Hirofumi, Imanishi, Takeshi
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Journal Title
Bioorganic & Medicinal Chemistry 14
Pages: 1029-1038
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Antineoplastic Agents. 551. Isolation and Structures of Bauhiniastatins 1-4 from Bauhinia purpurea.2006
Author(s)
Pettit, George R., Numata, Atsushi, Iwamoto, Chika, Usami, Yoshihide, Yamada, Takeshi, Ohishi, Hirofumi, Cragg, Gordon M
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Journal Title
Journal of Natural Products 69
Pages: 323-327
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Synthesis and properties of 2'-O,4'-C-methylene-oxy-methylene bridged nucleic acid.2006
Author(s)
Hari, Yoshiyuki, Obika, Satoshi, Ohnishi, Ryo, Eguchi, Ken, Osaki, Tomohisa, Ohishi, Hirofumi, Imanishi, Takeshi
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Journal Title
Bioorganic & Medicinal Chemistry 14
Pages: 1029-1038
Description
「研究成果報告書概要(欧文)」より
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