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2004 Fiscal Year Final Research Report Summary

Molecular Histo-cytochemical Analysis of Gene Expression Regulation by Artificial Control of Transcription Factors

Research Project

Project/Area Number 15590161
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionNagasaki University

Principal Investigator

IZUMI Shin-ichi  Nagasaki University, Graduate School of Biomedical Sciences, Instructor, 大学院・医歯薬学総合研究科, 助手 (40264246)

Co-Investigator(Kenkyū-buntansha) KOJI Takehiko  Nagasaki University, Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (30170179)
TAKANO Kunio  Nagasaki University, Graduate School of Biomedical Sciences, Professor (from 2003 through 2004), 大学院・医歯薬学総合研究科, 教授 (80050029)
HISHIKAWA Yoshitaka  Nagasaki University, Graduate School of Biomedical Sciences, Associate Professor, 大学院・医歯薬学総合研究科, 助教授 (60304276)
Project Period (FY) 2003 – 2004
KeywordsTranscription factor / Gene expression / Histochemistry / Estrogen / Prolactin / Pit-1 / DNA / Nucleus
Research Abstract

1.Effects of exogenously administered estrogenic substances on prolactin(PRL) gene expression through the transcription factors, estrogen receptors(ERs) and Pit-1,were examined. PRL-and Pit-1-positive cells were increased, whereas FSH/LH-positive cells were decreased in the pituitaries of mice treated with diethylstilbestrol(DES). ER α was localized in the nuclei of PRL and FSH/LH cells hi the control mice, whereas hi DES-treated mice ER α -positive PRL cells were decreased and ER β-positive PRL cells were induced and then increased instead. In ER α-knockout mice ER β was localized in PRL cell nuclei and population of ER β, PRL or FSH/LH positive cells was constant. Treatment of wild-type mice with DES seemed to increase PRL gene transcription with an increase of both Pit-1 and ER β as well as a decrease of ER α hi the PRL cell nuclei. It suggests that quantity and quality of the transcription factors are regulated by DES.
2.We examined effects of artificial regulation on intranuclear distribution of the transcription factors in PRL producing GH3 culture cells. Pit-1 and ERs were localized to clarify whether PRL gene transcription was repressed when the double-stranded nucleotide sequences of PRL gene regulatory element (1P), which bind to Pit-1, were trans-inducted into the nuclei to block the 1P-binding sites. An autoclave retrieval was effective to enhance signals from the nuclear Pit-1 and ERs detected by Southwestern histochemistry and peroxidase-labeled antibody method, respectively, both at light- and electron microscopic levels. GH3 cells, in which Pit-1 and ER α were localized in the euchromatin region in the vicinity of the heterochromatin, possessed PRL. It appeared that Pit-1 and ER were distributed hi a definite nuclear region where the gene is expressed in GH3 cells. Optimal concentration and tune after the probe trans-induction were under examination.

  • Research Products

    (20 results)

All 2005 2004 2003

All Journal Article (15 results) Book (5 results)

  • [Journal Article] Intense immuno-labeling of tryptophan 2,3-dioxygenase in the rat hepatocyte cytosol after treatment of cortisone and/or tryptophan2005

    • Author(s)
      Izumi S, Watanebe K
    • Journal Title

      Journal of Electron Microscopy Technology for Medicine and Biology 18(2)

      Pages: 69-75

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Realization of sites of specific molecules in the cell organelles : electron microscopic techniques for histochemical methods2005

    • Author(s)
      Izumi S
    • Journal Title

      Journal of Electron Microscopy Technology for Medicine and Biology 19(1)

      Pages: 107-109

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Realization of sites of specific molecules in the cell organelles : electron microscopic techniques for histo chemical methods2005

    • Author(s)
      Izumi S
    • Journal Title

      Journal of Electron Microscopy Technology for Medicine and Biology 19(1)

      Pages: 107-109

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Southwestern histochemistry : a method for localization of transcription factors.2005

    • Author(s)
      Izumi S, et al.
    • Journal Title

      Molecular morphology in human tissues : techniques and applications(CRC Press, Washington, D.C.)

      Pages: 133-146

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Immunohistochemical localization of the ACTH (MC-2) receptor in the rat placenta and adrenal gland2004

    • Author(s)
      Izumi S, et al.
    • Journal Title

      Archives of Histology and Cytology 67(5)

      Pages: 443-453

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Immunohistochemical identification of HSP47 and type I collagen-expressing cells in Mongolian gerbil salivary glands : correlation with age-related fibrotic changes2004

    • Author(s)
      Tomisaki H, et al.
    • Journal Title

      Oral Medicine and Pathology 9(4)

      Pages: 141-148

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] In situ hybridization of prolactin mRNA in rat pituitary sections : backscattered electron mode analysis using light microscopic specimen on glass slides2004

    • Author(s)
      Izumi S, et al.
    • Journal Title

      Advancement of Life Science(Amvo Publishing Company, Taipei)

      Pages: 153-158

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Molecular histochemistry of rRNAs by nonradioactive in situ hybridization at light- and electron-microscopic levels.2004

    • Author(s)
      Koji T, et al.
    • Journal Title

      Advancement of Life Science(Amvo Publishing Company, Taipei)

      Pages: 141-146

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Southwestern histochemical analysis of estrogen responsive element binding protein in paraffin-embedded sections of mouse ovary.2004

    • Author(s)
      Hishikawa Y, et al.
    • Journal Title

      Advancement of Life Science(Amvo Publishing Company, Taipei)

      Pages: 147-152

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Differential analysis of active and inactive genes in human neutrophils by chromosomal in situ hybridization2003

    • Author(s)
      Izumi S, et al.
    • Journal Title

      Acta Histochemica et Cytochemica 36(4)

      Pages: 325-330

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Molecular histochemical analysis of estrogen receptor a and β expressions in the mouse ovary : in situ hybridization and Southwestern histochemistry2003

    • Author(s)
      Hishikawa Y, et al.
    • Journal Title

      Medical Electron Microscopy 36

      Pages: 67-73

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 核内RNAの電顕in situ hybridizationによる検出技法2003

    • Author(s)
      末松貴史, 和泉伸一, 他
    • Journal Title

      医学生物学電子顕微鏡技術学会誌 17(1)

      Pages: 2-5

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Molecular histochemical analysis of estrogen receptor α and β expressions in the mouse ovary : in situ hybridization and Southwestern histochemistry2003

    • Author(s)
      Hishikawa Y, et al.
    • Journal Title

      Medical Electron Microscopy 36

      Pages: 67-73

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Electron microscopic in situ hybridization of Nuclear RNAs2003

    • Author(s)
      Suematsu T, Izumi S, et al.
    • Journal Title

      Journal of Electron Microscopy Technology for Medicine and Biology 17(1)

      Pages: 2-5

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] In situ hybridization for beginners.2003

    • Author(s)
      Izumi S, Koji T
    • Journal Title

      Histocytochemistry 2004(Gakusaikikaku, Tokyo)

      Pages: 133-146

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Southwestern histochemistry : a method for localization of transcription factors In : Molecular morphology in human tissues : techniques and applications2005

    • Author(s)
      Izumi S, et al.
    • Total Pages
      133-146
    • Publisher
      CRC Press, Washington, D.C
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] In situ hybridization of prolactin mRNA in rat pituitary sections : backscattered electron mode analysis using light microscopic specimen on glass slides. In : Advancement of Life Science2004

    • Author(s)
      Izumi S, et al.
    • Total Pages
      153-158
    • Publisher
      Amvo Publishing Company, Taipei
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] Molecular histochemistry of rRNAs by nonradioactive in situ hybridization at light- and electron-microscopic levels. In : Advancement of Life Science2004

    • Author(s)
      Koji T, et al.
    • Total Pages
      141-146
    • Publisher
      Amvo Publishing Company, Taipei
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] Southwestern histochemical analysis of estrogen responsive element binding protein in paraffin-embedded sections of mouse ovary, In : Advancement of Life2004

    • Author(s)
      Hishikawa Y, et al.
    • Total Pages
      147-152
    • Publisher
      Amvo Publishing Company, Taipei
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 初心者のためのin situ hybridization法、「組織細胞化学2004」2003

    • Author(s)
      和泉伸一, 小路武彦
    • Total Pages
      155-160
    • Publisher
      学際企画、東京
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2006-07-11  

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