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2005 Fiscal Year Final Research Report Summary

Identification and functional analyses of CO_2-sensing cells in the central respiratory chemoreceptor

Research Project

Project/Area Number 15590190
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General physiology
Research InstitutionKeio University

Principal Investigator

OKADA Yasumasa  Keio University, Department of Medicine, Associate Professor, 医学部, 助教授 (80160688)

Co-Investigator(Kenkyū-buntansha) KUWANA Shun-ichi  Teikyo University, Department of Medicine, Assistant Professor, 医学部, 講師 (70129998)
Project Period (FY) 2003 – 2005
Keywordsrespiratory control / central chemosensitivity / propofol / brainstem / respiratory neuronal network / voltage imaging / GABA / muscle relaxant
Research Abstract

In this research project we have studied the function and anatomy of the neural substrate for the central respiratory chemoreception and central respiratory control.
In order to identify the functional role of an important neurotransmitter GABA in central respiratory control, we used GABA-synthesizing enzyme (GAD) gene-targeted mice, and found that GABA is necessary in the maintenance of stable respiratory output (Kuwana et al. 2003 ; 2004 ; 2006). We also found that respiratory depression by propofol is induced by its GABA-like inhibitory action (Kashiwagi et al. 2004 ; 2004).
We applied a voltage imaging technique to an in vitro brainstem preparation, and succeeded functional mapping of chemosensitive regions. We reported that the pontine A5 region has intrinsic chemosensitivity (Ito et al. 2004).
On the basis of our hypothesis that nicotinic acetylcholine receptor is importantly involved in central respiratory chemoreception, we analyzed the effects of non-depolarizing muscle relaxants on chemoresponsiveness in vitro. We first reported that non-depolarizing muscle relaxants inhibit respiratory activity in the brainstem (Sakuraba et al. 2003 ; 2005).
We summarized the results of our studies, and have proposed our theories that potassium channels play a key role as the chemosensor in chemoreceptor cells (Oyamada et al. 2006) and that specific small cells surrounding fine vessels in the superficial ventral medulla are primary chemoreceptor cells (Okada et al. 2006).

  • Research Products

    (37 results)

All 2006 2005 2004 2003

All Journal Article (35 results) Book (2 results)

  • [Journal Article] Electrophysiological and morphological characteristics of GABAergic respiratory neurons in the mouse pre-Botzinger complex2006

    • Author(s)
      Kuwana S
    • Journal Title

      European Journal of Neuroscience 23・3

      Pages: 667-674

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Ischemia-induced disturbance of neural network function in the rat spinal cord analyzed by voltage-imaging2006

    • Author(s)
      Fukuda K
    • Journal Title

      Neuroscience (in press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Potassium channels in the central control of breathing2006

    • Author(s)
      Oyamada Y
    • Journal Title

      Advances in Experimental Medicine and Biology 580

      Pages: 339-344

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The cell-vessel architecture model of central respiratory chemoreceptor2006

    • Author(s)
      Okada Y
    • Journal Title

      Advances in Experimental Medicine and Biology 580

      Pages: 233-238

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Electrophysiological and morphological characteristics of GABAergic respiratory neurons in the mouse pre-Botzinger complex2006

    • Author(s)
      Kuwana S
    • Journal Title

      European Journal of Neuroscience 23(3)

      Pages: 667-674

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Significance of extracellular potassium in central respiratory control studied in the isolated brainstem-spinal cord preparation of the neonatal rat2005

    • Author(s)
      Okada Y
    • Journal Title

      Respiratory Physiology & Neurobiology 146・1

      Pages: 21-32

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Effects of neuromuscular blocking agents on central respiratory chemosensitivity in newborn rats2005

    • Author(s)
      Sakuraba S
    • Journal Title

      Biological Research 38・2-3

      Pages: 225-233

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] GABA合成酵素遺伝子ノックアウトマウスの呼吸障害2005

    • Author(s)
      桑名俊一
    • Journal Title

      Clinical Neuroscience 23・3

      Pages: 348-348

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 呼吸中枢2005

    • Author(s)
      岡田泰昌
    • Journal Title

      臨床麻酔 29・3

      Pages: 573-583

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 呼吸調節メカニズムの最近の知見2005

    • Author(s)
      岡田泰昌
    • Journal Title

      呼吸と循環 53・5

      Pages: 523-530

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Significance of extracellular potassium in central respiratory control studied in the isolated brainstem-spinal cord preparation of the neonatal rat2005

    • Author(s)
      Okada Y
    • Journal Title

      Respiratory Physiology & Neurobiology 146(1)

      Pages: 21-32

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Effects of neuromuscular blocking agents on central respiratory chemosensitivity in newborn rats2005

    • Author(s)
      Sakuraba S
    • Journal Title

      Biological Research 38(2-3)

      Pages: 225-233

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Respiratory disturbance in GABA synthesizing enzyme gene knockout mice2005

    • Author(s)
      Kuwana S
    • Journal Title

      Clinical Neuroscience (in Japanese) 23(3)

      Pages: 348-348

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Respiratory center2005

    • Author(s)
      Okada Y
    • Journal Title

      Rinshomasui (in Japanese) 29(3)

      Pages: 573-583

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Recent progress in the study of respiratory control mechanisms2005

    • Author(s)
      Okada Y
    • Journal Title

      Kokyutojunkan (in Japanese) 53(5)

      Pages: 523-530

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A neuronal mechanism of propofol-induced central respiratory depression in neonatal rats2004

    • Author(s)
      Kashiwagi M
    • Journal Title

      Anesthesia & Analgesia 99・1

      Pages: 49-55

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Electrical stimulation of the rabbit pulmonary artery increases respiratory output2004

    • Author(s)
      Okada Y
    • Journal Title

      Respiratory Physiology & Neurobiology 140・3

      Pages: 209-217

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Functional connection from the surface chemosensitive region to the respiratory neuronal network in the rat medulla2004

    • Author(s)
      Okada Y
    • Journal Title

      Advances in Experimental Medicine and Biology 551

      Pages: 45-51

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Role of GABA in respiratory control studied using mice lacking GABA-synthesizing enzyme 67-kDa isoform of glutamic acid decarboxylase2004

    • Author(s)
      Kuwana S
    • Journal Title

      Advances in Experimental Medicine and Biology 551

      Pages: 157-163

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Mechanism of propofol-induced central respiratory depression in neonatal rats2004

    • Author(s)
      Kashiwagi M
    • Journal Title

      Advances in Experimental Medicine and Biology 551

      Pages: 221-226

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Mixed venous CO_2 and ventilation during exercise and CO_2-rebreathing in humans2004

    • Author(s)
      Satoh T
    • Journal Title

      Advances in Experimental Medicine and Biology 551

      Pages: 269-274

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Optical mapping of pontine chemosensitive regions of neonatal rat2004

    • Author(s)
      Ito Y
    • Journal Title

      Neuroscience Letters 366・1

      Pages: 103-106

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 運動制限を伴う呼吸器疾患2004

    • Author(s)
      岡田泰昌
    • Journal Title

      月刊薬事 46・4(臨時増刊号)

      Pages: 215-222

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 呼吸困難感と呼吸調節2004

    • Author(s)
      岡田泰昌
    • Journal Title

      medicina 41・7

      Pages: 1096-1101

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A neuronal mechanism of propofol-induced central respiratory depression in neonatal rats2004

    • Author(s)
      Kashiwagi M
    • Journal Title

      Anesthesia &Analgesia 99(1)

      Pages: 49-55

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Electrical stimulation of the rabbit pulmonary artery increases respiratory output2004

    • Author(s)
      Okada Y
    • Journal Title

      Respiratory Physiology & Neurobiology 140(3)

      Pages: 209-217

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Optical mapping of pontine chemosensitive regions of neonatal rat2004

    • Author(s)
      Ito Y
    • Journal Title

      Neuroscience Letters 366(1)

      Pages: 103-106

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Respiratory diseases with exercise limitation2004

    • Author(s)
      Okada Y
    • Journal Title

      Gekkan Yakuji (in Japanese) 46(4)

      Pages: 215-222

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Dyspneic sensation and respiratory control2004

    • Author(s)
      Okada Y
    • Journal Title

      medicina (in Japanese) 41(7)

      Pages: 1096-1101

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Disturbance of neural respiratory control in neonatal mice lacking GABA synthesizing enzyme 67-kDa isoform of glutamic acid decarboxylase2003

    • Author(s)
      Kuwana S
    • Journal Title

      Neurosocience 120・3

      Pages: 861-870

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Effects of neuromuscular blocking agents on central respiratory control in the isolated brainstem-spinal cord of the neonatal rat2003

    • Author(s)
      Sakuraba S
    • Journal Title

      Neuroscience Research 47・3

      Pages: 289-298

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Synaptic blockade plays a major role in the neural disturbance of experimental spinal cord compression2003

    • Author(s)
      Yoshida H
    • Journal Title

      Journal of Neurotrauma 20・12

      Pages: 1365-1376

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Disturbance of neural respiratory control in neonatal mice lacking GABA synthesizing enzyme 67-kDa isoform of glutamic acid decarboxylase2003

    • Author(s)
      Kuwana S
    • Journal Title

      Neurosocience 120(3)

      Pages: 861-870

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Effects of neuromuscular blocking agents on central respiratory control in the isolated brainstem-spinal cord of the neonatal rat2003

    • Author(s)
      Sakuraba S
    • Journal Title

      Neuroscience Research 47(3)

      Pages: 289-298

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Synaptic blockade plays a major role in the neural disturbance of experimental spinal cord compression2003

    • Author(s)
      Yoshida H
    • Journal Title

      Journal of Neurotrauma 20(12)

      Pages: 1365-1376

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] 呼吸の事典2006

    • Author(s)
      桑名俊一
    • Total Pages
      728
    • Publisher
      朝倉書店
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] Kokyunojiten (Respiration and Gene Targeting) (Arita H ed.) (in Japanese)2006

    • Author(s)
      Kuwana S
    • Total Pages
      728
    • Publisher
      Asakurashoten (Tokyo)
    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2007-12-13  

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