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2005 Fiscal Year Final Research Report Summary

Nuclear translocation of antioxidant enzymes and ROS metabolism

Research Project

Project/Area Number 15590258
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General medical chemistry
Research InstitutionHyogo College of Medicine

Principal Investigator

SUZUKI Keiichirou  Hyogo College of Medicine, Faculty of Medicine, Professor, 医学部, 教授 (70221322)

Co-Investigator(Kenkyū-buntansha) OOKAWARA Tomomi  Hyogo College of Medicine, Faculty of Medicine, Assistant Professor, 医学部, 講師 (50330452)
FUJIWARA Noriko  Hyogo College of Medicine, Faculty of Medicine, Assistant Professor, 医学部, 講師 (10368532)
EGUCHI Hironobu  Hyogo College of Medicine, Faculty of Medicine, Research Associate, 医学部, 助手 (60351798)
KUROTSU Tositsugu  Hyogo College of Medicine, Faculty of Medicine, Associate Professor, 医学部, 助教授 (20098528)
Project Period (FY) 2003 – 2005
KeywordsROS / superoxside dismutase / EC-SOD / oxidative stress / arteriosclerosis / HB-EGF
Research Abstract

Extracellular superoxide dismutase (EC-SOD) is the only known secretory SOD isoenzyme. EC-SOD has a positively-charged heparin-binding domain at the C-terminus and it has been thought that this domain and the secretory nature could allow the enzyme characteristic multiple distributions after the secretion.
1.We have purified recombinant EC-SOD protein from the cultured medium of the stably mouse EC-SOD expressing cell line, CHO-EK.
2.Physical training apparently decreased EC-SOD level in humam plasma as a reflection of the increased binding capacity in tissues to EC-SOD and heavy physical examination, which was simulated the ischemic-reperfusion injuries, brought a release of tissue bound EC-SOD to the blood stream.
3.3T3-L1 cells incorporated EC-SOD from the medium and a significant fraction of the material taken up was localized in the nucleus. Oxidative stress on the 3T3-L1 cell downregulated the uptake of EC-SOD and the nuclear translocation of the incorporated EC-SOD was clearly enhanced by H_2O_2 treatment.
4.EC-SOD purified from the human lung tissue was fractionated through heparin affinity chromatography and clarified the relation between the modification at C-terminus and heparin affinity.
5.Investigation using RASMC and the recombinant EC-SOD revealed that RASMC incorporated EC-SOD from the medium and heparin interfered the binding of EC-SOD to RASMC. Moreover, TPA and hydrogen peroxide induced the production of ROS in RASMC and gene expression of HB-EGF and pre-treatment of the RASMC with EC-SOD suppressed the induction of HB-EGF induction, suggesting that EC-SOD inhibited the EGF signaling evoked by ROS stimulation in RASMC.

  • Research Products

    (18 results)

All 2006 2005 2003

All Journal Article (18 results)

  • [Journal Article] Inhibition of gene expression of heparin-binding epidermal growth factor-like growth factor by extracellular superoxide dismutase in rat smooth muscle cells2006

    • Author(s)
      Nishimura, M.
    • Journal Title

      Free Radic Res (In press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Reactive oxygen species modify oligosaccharides of glycoproteins in vivo : a study of a spontaneous acute hepatitis model rat (LEC rat).2006

    • Author(s)
      Yasuda, J.
    • Journal Title

      Biochem Biophys Res Coommun 342・1

      Pages: 127-134

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Inhibition of gene expression of heparin-binding epidermal growth factor-like growth factor by extracellular superoxide dismutase in rat smooth muscle cells2006

    • Author(s)
      Nishimura, M.
    • Journal Title

      Free Radic Res. (In press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Reactive oxygen species modify oligosaccharides of glycoproteins in vivo : a study of a spontaneous acute hepatitis model rat (LEC rat).2006

    • Author(s)
      Yasuda, J.
    • Journal Title

      Biochem Biophys Res Coommun. 342.1

      Pages: 127-134

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Tumor necrosisi factor-a-induced iron sequestration and oxidative stress in human endothelial cells.2005

    • Author(s)
      Nanami, M
    • Journal Title

      Arterioscler Thromb Vase Biol 25・12

      Pages: 2495-501

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Modification of oligosaccharides by reactive oxygen species decreases sialyl lewis x mediated cell adhesion.2005

    • Author(s)
      Eguchi, H.
    • Journal Title

      Glycobiology 15・11

      Pages: 1094-101

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Different immunoreactivity against monoclonal antibodies between wild-type and mutant copper/zinc superoxide dismutase linked to amyotrophic lateral sclerosis.2005

    • Author(s)
      Fujiwara, N.
    • Journal Title

      J Biol Chem 280・6

      Pages: 5061-70

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Overexpression of mutated Cu,Zn-SOD in neuroblastoma cells results in cytoskeletal change.2005

    • Author(s)
      Takamiya, R.
    • Journal Title

      Am J Physiol Cell Physiol 288・2

      Pages: C253-9

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Tumor necrosisi factor-a-induced iron sequestration and oxidative stress in human endothelial cells.2005

    • Author(s)
      Nanami, M.
    • Journal Title

      Arterioscler Thromb Vasc Biol. 25.12

      Pages: 2495-2501

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Modification of oligosaccharides by reactive oxygen species decreases sialyl lewis x mediated cell adhesion.2005

    • Author(s)
      Eguchi, H.
    • Journal Title

      Glycobiology 15.11

      Pages: 1094-1101

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Different immunoreactivity against monoclonal antibodies between wild-type and mutant copper/zinc superoxide dismutase linked to amyotrophic lateral sclerosis.2005

    • Author(s)
      Fujiwara, N.
    • Journal Title

      J Biol Chem. 280.6

      Pages: 5061-5070

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Overexpression of mutated Cu, Zn-SOD in neuroblastoma cells results in cytoskeletal change.2005

    • Author(s)
      Takamiya, R.
    • Journal Title

      Am J Physiol Cell Physiol 288.2

      Pages: 253-259

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Effects of endurance training on three Superoxide dismutase isoenzymes in human plasma.2003

    • Author(s)
      Ookawara, T.
    • Journal Title

      Free Radic. Res. 372

      Pages: 713-719

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Effects of oxidative stress on the nuclear translocation of extracellular superoxide dismutase.2003

    • Author(s)
      Ookawara, T.
    • Journal Title

      Biochem. Biophys. Res. Commun. 303

      Pages: 914-919

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] An inter-subunit disulfide bond affects affinity of human lung extracellular superoxide dismutase to heparin.2003

    • Author(s)
      Ookawara, T.
    • Journal Title

      Free Radic. Res. 37

      Pages: 823-827

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Effects of endurance training on three Superoxide dismutase isoenzymes in human plasma.2003

    • Author(s)
      Ookawara, T.
    • Journal Title

      Free Radic.Res. 372

      Pages: 713-719

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Effects of oxidative stress on the nuclear translocation of extracellular superoxide dismutase.2003

    • Author(s)
      Ookawara, T.
    • Journal Title

      Biochem.Biophys.Res.Commun. 303

      Pages: 914-919

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] An inter-subunit disulfide bond affects affinity of human lung extracellular superoxide dismutase to heparin.2003

    • Author(s)
      Ookawara, T.
    • Journal Title

      Free Radic.Res. 37

      Pages: 823-827

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2007-12-13  

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