2004 Fiscal Year Final Research Report Summary
Study of nematode mitochondrial translation system using parasitic nematodes
| Project/Area Number |
15590365
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Parasitology (including Sanitary zoology)
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
WATANABE Yoh-ichi The University of Tokyo, Graduate School of Medicine, Lecturer, 大学院・医学系研究科, 講師 (90323568)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
OHTSUKI Takashi Okayama University, Faculty of Engineering, Lecturer, 工学部, 講師 (80321735)
KITA Kiyoshi The University of Tokyo, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (90134444)
KAMIYA Haruo Hirosaki University, Faculty of Medicine, Professor, 医学部, 教授 (70002079)
|
|---|
| Project Period (FY) |
2003 – 2004
|
| Keywords | nematode / mitochondria / translation / tRNA / elongation factor Tu / Caenorhabditis elegans / Trichinella britovi |
|---|
| Research Abstract |
Most nematode mitochondria have D-armless tRNAs, which also exist in most metazoan mitochondria, and T-armless tRNAs. On the other hand, bacterial translation elongation factor Tu (EF-Tu) needs T-arm of aminoacyl-tRNA for the complex formation. In order to investigate how nematode mitochondrial EF-Tu recognizes the T-armless tRNA, we cloned EF-Tus from various nemaotdes. Nematode have two distinct EF-Tus. Two EF-Tus, namely EF-Tu1 and EF-Tu2, have novel C-terminal extensions (about 40-60 residues and about 15 residues, respectively) when compared with bacterial EF-Tu. Caernarhabditis elegans and Trichinella britovi EF-Tu1 bound to the T-armless tRNAs, while EF-Tu2 bound to D-armless tRNAs. C.elegans EF-Tus and T.britovi EF-Tu2 could not bind to conventional tRNAs, while T.britovi EF-Tu1 could bind to conventional tRNAs. These results consist well with the fact that C.elegans mitochondria do not have any conventional tRNA but Trichinella mitochondria have. Deletion of 10 residues of C-terminus of both of C.elegans EF-Tus lead to loss of binding to the D-armless or the T-armless tRNAs, suggesting the novel C-Terminal extensions may involve in interaction between EF-Tus and the truncated nematode tRNAs. In summary, the nematode mitochondrial EF-Tu adapted to the deletion of D-arm or T-arm of tRNAs by the gene duplication and aquirment of novel C-terminal extension to make a new interaction between the truncated tRNA and EF-Tu.
|
