2005 Fiscal Year Final Research Report Summary
Ultrastructural Analysis of Mechanism of Protein-Domain-Assembly on Cell Membrane of Specific Lipid-Binding Toxin.
| Project/Area Number |
15590396
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Kitasato University |
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Principal Investigator |
SEKIYA Kachiko Kitasato Univ., Sch. of Pharm., Assistant Prof., 薬学部, 講師 (30050579)
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| Co-Investigator(Kenkyū-buntansha) |
ABE Akio Kitasato Univ., Kitasato Institute for Life Sciences, Prof., 北里生命科学研究所, 教授 (50184205)
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| Project Period (FY) |
2003 – 2005
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| Keywords | Streptolysin O / Hemolysis / Electron Microscopy / Negative staining / SLO / cholesterol / Protein-domain / Pore-forming Toxin |
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| Research Abstract |
Streptolysin O (SLO) is a membrane-damaging toxic protein produced by group A streptococci. An ultrastructural analysis of pore formation and the mechanism of hemolysis by SLO, using a mutant form of SLO [SLO(C/A)-SS and native SLO. Electron micrographs of negatively stained preparations revealed that SLO(C/A)-SS was unable to penetrate the erythrocyte membrane as a consequence of immobilization that was due to a disulfide bond between domains. The SLO(C/A)-SS molecules that bound to membranes formed numerous single-layered ring-shaped structures that did not result in pores on the membranes. These structures were similar to the structures formed by native SLO at 0℃. After treatment with dithiothreitol (DTT), SLO(C/A)-SS that had bound to membranes formed double-layered rings with pores on the membranes, as does native SLO at room temperature. There was a correlation between the number of double-layered ring structure with pores that formed and the duration of treatment with DTT. From these results, the following two points were concluded ; (1) Each inner and outer layer of the previously proposed ring model of SLO (J.Bacteriol. 1993) was composed of single SLO molecules associated in a circle. (2) Hemolysis by SLO toxin involves two defined steps; a temperature-independent step and a temperature-dependent step. An increase in temperature is necessary for the occurrence of conformational changes and the formation of the double-layered ring after the insertion of domain 3 into the host cell membrane.
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Research Products
(9 results)
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[Journal Article] Assembly of the type III secretion apparatus of enteropathogenic Escherichia coli.2006
Author(s)
Ogino, T., Ohno, R., Sekiya, K., Kuwae, A., Matsuzawa, T., Abe, A.et al.
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Journal Title
J. Baceriol. 188(8)
Pages: 2801-2811
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Assembly of the type III secretion apparatus of enteropathogenic Escherichia coll.2006
Author(s)
Ogino, T., Ohno, R., Sekiya, K., Kuwae, A., Matsuzawa, T., Nonaka, T., Fukuda, H., Imajoh-Ohmi, S., Abe, A.
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Journal Title
J.Baceriol. 188(8)
Pages: 2801-2811
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Azithromycin inhibits the formation of flagellar filaments without suppressing flagellin synthesis in Salmonella enterica serovar Typhimurium.2005
Author(s)
Matsui, H., Eguchi, M., Ohsumi, K., Isshiki, Y., Sekiya, K., Kikuchi, Y., et al.
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Journal Title
Antimicrob. Agents Chemother. 49(8)
Pages: 3396-3403
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Nocardioides aromaticivorans sp.nov., a dibenzofuran-degrading bacterium isolated from dioxin-polluted environments.2005
Author(s)
Kubota, M., Kawahara, K., Sekiya, K., Uchida, T., Hattori, Y., Futamata, H., Hiraishi, A.
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Journal Title
Systematic Applied Microbiol. 28
Pages: 165-174
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A zithromycin inhibits the formation of flagellar filaments without suppressing flagellin synthesis in Salmonella enterica serovar Typhimurium.2005
Author(s)
Matsui, H., Eguchi, M., Ohsumi, K., Nakamura, A., Isshiki, Y., Sekiya, K., Kikuchi, Y., Nagamitsu, T., Masuma, R., Sunazuka, T., Omura, S.
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Journal Title
Antimicrob.Agents Chemother. 49(8)
Pages: 3396-3403
Description
「研究成果報告書概要(欧文)」より
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[Book] 基礎病原微生物学2005
Author(s)
檀原宏文, 田口文章, 関矢加智子ら20名
Total Pages
835
Publisher
廣川書店
Description
「研究成果報告書概要(和文)」より
