2004 Fiscal Year Final Research Report Summary
A mutant of Escherichia coli enterotoxin inducing a specific Tb1-type of T cells to varicella-zoster vaccine enhances the production of IL-12 by IFNγ-stimulated macrophages
| Project/Area Number |
15590403
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Fujita Health University |
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Principal Investigator |
TSUJI Takao Fujita Health University, School of Medicine, Professor, 医学部, 教授 (60171998)
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| Co-Investigator(Kenkyū-buntansha) |
OCHI Sadayuki Fujita Health University, School of Medicine, Assistant Professor, 医学部, 講師 (80268705)
ARIMITSU Hideyuki Fujjita Health University, School of Medicine, Research Associate, 医学部, 助手 (40367701)
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| Project Period (FY) |
2003 – 2004
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| Keywords | mucosal adjuvant / eat-labile enterotoxin / cellular immunity / mutant / Th1 / Th2 balance / macrophage / IL-12 / TNF α |
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| Research Abstract |
A mutant of Escherchia colt enterotoxin induces specific Th1-type T cells to varicella-zoster vaccine. The mutant increased IL-12p40, TNFα and nitric oxide production by IFNγ -stimulated bone marrow macrophages but cholera toxin did not. Anti-TNFα antibodies blocked its stimulation of IL-12p40 production but iNOS inhibitor did not. IL-12p40 and IL-12p35 production was stimulated at the level of mRNA formation by the mutant. Cholera toxin suppressed IL-12(31 expression by spleen T cells stimulated with anti-CD3 antibodies but the mutant did not. These findings indicate that the mutant may induce Th 1-type response to the vaccine through its IL-12 and TNFα induction by macrophages.
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