2004 Fiscal Year Final Research Report Summary
Identification of cellular receptor and its ligand for human herpesvirus 6
| Project/Area Number |
15590417
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Osaka University |
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Principal Investigator |
MORI Yasuko Osaka University, Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (50343257)
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| Co-Investigator(Kenkyū-buntansha) |
YAMANISHI Koichi Osaka University, Graduate School of Medicine, Professor, 医学系研究科, 教授 (10029811)
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| Project Period (FY) |
2003 – 2004
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| Keywords | HHV-6 / CD46 / tropism / gH / gL / gQ1 / gQ2 / gH / gL / gO / viral glycoproteins / virions / ligand |
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| Research Abstract |
We have studied about the cellular receptors and the ligand(s) of human herpes virus 6 (HHV-6). During the time, we found as follows.
(1)HHV-6 gQ gene encodes an additional product whose mature molecular mass is 37kDa (gQ-37K), and which is derived from a different transcript.
(2)We designated gQ-80K as gQl and gQ37K as gQ2.
(3)The gQ2 also interacts with the gH/gL/gQ1 complex in HHV-6 infected cells and virions.
(4)The gQ2 and gQ1 interact in the endoplasmic reticulum, however, gH/gL/gQ1/gQ2 heterotetrameric complex arises in post endoplasmic reticulum compartment, and the mature complex is subsequently incorporated into viral particles.
(5)The heterotetrameric complex is viral ligand for HHV-6 cellular receptor, human CD46.
(6)HHV-6 gO gene encodes a third component of HHV-6 gH/gL containing envelope complex.
(7)The gH/gL/gO complex does not bind to human CD46, indicating that the complex is not ligand for human CD46.
(8)The gH/gL/gO and gH/gL/gQ1/gQ2 complexes express in virions independently.
(9)These results indicate that the gH/gL/gO complex may play a role different from that of gH/gL/gQ1/gQ2 during viral infection.
(10)This is a first report of two kinds of gH/gL complexes on the viral envelope in a member of the herpesvirus family.
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