2004 Fiscal Year Final Research Report Summary
Analysis of packaging mechanism of influenza virus genome and the applications of viral vector
| Project/Area Number |
15590419
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Kawesaki Medical School (2004)
Hiroshima University (2003) |
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Principal Investigator |
FUJII Yutaka Kawasaki Medical school, Microbiology, Associate Professor, 医学部, 助教授 (30274054)
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| Project Period (FY) |
2003 – 2004
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| Keywords | influenza virus / genome / selective incorporation / base pairing model / vector / ベクター |
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| Research Abstract |
The genome of influenza A virus is comprised of eight viral RNA (vRNA) segments. Although the products of all eight vRNA segments must be present for viral replication, little is known about the mechanism(s) responsible for incorporation of these segments into viricns. Two models have proposed for the generation of infectious virions containing eight vRNA segments. The random-incorporation model assumes a common structural feature in all the vRNAs, enabling any combination of vRNAs to be imported randomly into virions. The selective-incorporation model predicts the presence of specific structures in each vRNA segment, leading to the incorporation of a set of eight vRNA segments into virions. We demonstrate the eight different vRNA segments must be present for efficient virion formation and that sequences within the coding region of the neuraminidase vRNA possess a signal that drives incorporation of this segment into virions. These findings indicate a unique contribution from individual vRNA segments and thus suggest a selective mechanism of vRNA recruitment into virions. We used the signals to incorporate foreign genes into influenza. Because the genes are expressed effectively in the infected cells, we thought this is a good method to use influenza virus as an expressing vector.
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