2004 Fiscal Year Final Research Report Summary
Study of mtDNA analysis from mixed stains used by cloning techniques
| Project/Area Number |
15590580
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Legal medicine
|
|---|
| Research Institution | Faculty of Medicine (Miyazaki Medical College), University of miyazaki (2004)
宮崎医科大学 (2003) |
|---|
Principal Investigator |
SEO Yasuhisa University of Miyazaki, Faculty of Medicine, Associate Professor, 医学部, 助教授 (80187830)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YUKAWA Nobuhiro University of Miyazaki, Faculty of Medicine, Professor, 医学部, 教授 (30240154)
TAKAMI Yasunari University of Miyazaki, Faculty of Medicine, Associate Professor, 医学部, 助教授 (80236356)
KAKIZAKI Eiji University of Miyazaki, Faculty of Medicine, Assistant Professor, 医学部, 助手 (70284833)
|
|---|
| Project Period (FY) |
2003 – 2004
|
| Keywords | Personal identification / DNA polymorphism / Mitochondrion / mtDNA / Sequence / Cloning / PCR |
|---|
| Research Abstract |
Mitochondrial DNA(mtDNA) has a chain structure, and it is different from nuclear DNA which consists of homologous chromosomes. The PCR product of mtDNA is usually produced by the origin for the single arrangement as for the mtDNA. When analyzing mixed stains, it is difficult to identify for each STR types. However, PCR products of mtDNA are easily distinguishable by using cloning techniques. 9 samples of mixed stain, which are contaminated by saliva and/or blood from two or three volunteer, are prepared and DNA is extracted from these subjects. Usual mtDNA typing has done by sequence analysis, and then PCR products are inserted into E.Coli. A cloning segment in each colony is amplified by colony-PCR and sequencing. As results, multiple peak location is observed in the electoropherogram when analyzed primary PCR products. It is possible to distinguish and identify in each PCR product from cloning samples. Therefore, cloning analysis of mtDNA is very useful for distinguishing mixed satin samples and identifying individuals.
|
