2003 Fiscal Year Annual Research Report

メチル化阻害剤・酸分泌抑制剤・COX2阻害剤のBarrett粘膜分化誘導作用の検討

Research Project

Project/Area Number	15590664
Research Institution	Sapporo Medical University
Principal Investigator	遠藤高夫札幌医科大学, 医学部, 助教授 (40191928)
Co-Investigator(Kenkyū-buntansha)	山本博幸札幌医科大学, 医学部, 助手 (40332910)
Keywords	Barrett粘膜 / 細胞増殖能 / 酸分泌抑制剤
Research Abstract	1)生検で得られたBarrett粘膜を3分割し,一方を組織学的ならびに免疫組織学的検討を行った.他方は95%O_2 5%CO_2 37℃下で10%FCS加培養液にて器官培養を行った.酸曝露は0.1mol/LHCl添加によりpH4.0条件で1時間曝露後中性環境に戻したところ.酸曝露前後で転写因子SOXは低下,CDX2,COX2は増加傾向を示し,COX1は不変であった.これらの傾向は統計学的に有意とはならなかったが,バラツキはBarrett粘膜の組織学的heterogenietyに起因する可能性は否定できない.一方BrdUによる細胞増殖能は酸曝露後に有意に上昇した. 2)Narrow Band Imaging(NBI)内視鏡と通常内視鏡によるBarrett粘膜の観察能を定量的に評価した.NBI内視鏡は通常内視鏡よりpit構造の描出能が有意に優れ,特に微小な再生扁平上皮島(micro-squamous island)ならびに粘膜表層毛細血管網を検出する手段として特段の有用性が証明された.そこで酸分泌抑制剤投与前後(6ヶ月間)のBarrett粘膜性状についてNBI内視鏡により観察を行い粘膜pit-pattern, vasvular network pattern, Barrett粘膜長,ならびに新たに出現した重層扁平上皮島の数について検討した.pit pattern, vasvular network pattern, Barrett粘膜長にはほとんど変化を認めなかったが,重層扁平上皮島の数は有意に増加した.粘液組成の変化についてはMUC5AC, MUC6の発現増加ならびにsulfo-Le^a詳sialylTnの発現減少傾向を認めた.また前後でのアポトーシス関連蛋白の変化は認められなかった.

Research Products

(6 results)

All Other

All Publications (6 results)

[Publications] Hamamoto Y, Endo T: "Usefulness of narrow-band imaging endoscopy for diagnosis of Barrett's esophagus."J Gastroenterol. 39. 14-20 (2004)
[Publications] Satoh A, Toyota M, Endo T et al.: "Epigenetic inactivation of CHFR and sensitivity to microtubule inhibitors in gastric cancer."Cancer Res. 63. 8606-8613 (2003)
[Publications] Matsuno K, Adachi Y, Endo T et al.: "The expression of matrix metalloproteinase matrilysin indicates the degree of inflammation in ulcerative colitis."J Gastroenterol. 38. 348-354 (2003)
[Publications] Yamashita K, Arimura Y, Endo T. et al.: "Increased cyclooxygenase-2 expression in large flat colorectal tumors (laterally spreading tumors)."J Gastroenterol. 38. 69-73 (2003)
[Publications] Gono K, Yamazaki K, Endo T. et al.: "Endoscopic observation of tissue by Narrow band illumination."Optical rev. 10. 211-215 (2003)
[Publications] Gono K, Obi T, Endo T, et al.: "Appearance of enhanced tissue features in narrow-band endoscopic imaging."Journal of Biomedical Optics. 9. 568-577 (2004)

2003 Fiscal Year Annual Research Report

メチル化阻害剤・酸分泌抑制剤・COX2阻害剤のBarrett粘膜分化誘導作用の検討

Principal Investigator

遠藤 高夫 札幌医科大学, 医学部, 助教授 (40191928)

Research Products

[Publications] Hamamoto Y, Endo T: "Usefulness of narrow-band imaging endoscopy for diagnosis of Barrett's esophagus."J Gastroenterol. 39. 14-20 (2004)

[Publications] Satoh A, Toyota M, Endo T et al.: "Epigenetic inactivation of CHFR and sensitivity to microtubule inhibitors in gastric cancer."Cancer Res. 63. 8606-8613 (2003)

[Publications] Matsuno K, Adachi Y, Endo T et al.: "The expression of matrix metalloproteinase matrilysin indicates the degree of inflammation in ulcerative colitis."J Gastroenterol. 38. 348-354 (2003)

[Publications] Yamashita K, Arimura Y, Endo T. et al.: "Increased cyclooxygenase-2 expression in large flat colorectal tumors (laterally spreading tumors)."J Gastroenterol. 38. 69-73 (2003)

[Publications] Gono K, Yamazaki K, Endo T. et al.: "Endoscopic observation of tissue by Narrow band illumination."Optical rev. 10. 211-215 (2003)

[Publications] Gono K, Obi T, Endo T, et al.: "Appearance of enhanced tissue features in narrow-band endoscopic imaging."Journal of Biomedical Optics. 9. 568-577 (2004)

遠藤高夫札幌医科大学, 医学部, 助教授 (40191928)