2005 Fiscal Year Final Research Report Summary
Alterations of potassium channels in vascular smooth muscle cells and endothelial cells in hypertension
| Project/Area Number |
15590853
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | University of the Ryukyus |
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Principal Investigator |
OHYA Yusuke University of tha Ryukyus, Faculty of Medicine, Associate Professor, 医学部, 助教授 (30240964)
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| Project Period (FY) |
2003 – 2005
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| Keywords | Ion channels / Hypertension / Potassium / NADPH oxidase / Oxidative Stress |
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| Research Abstract |
Voltage dependent potassium currents in endothelial cells were decreased in hypertensive model rats such as SHR and SHR-SP. We previously reported that this alteration was partially due to the decreased expression of Kv 1.5 channel protein. We also observed that antihypertensive treatment on hypertensive rats restored this alteration. We thus hypothesize that renin-angiotensin system plays an important role on the control of potassium channel activities. In the present study, we observed that potassium currents were decreased in mesenteric smooth muscle cells from SHR, as compared to WKY. mRNA expression of Kv2.1 was decreased in SHR, but that of Kv1.5 and Kv1.2 remained the same as compared to WKY. An extracellular oxidative stress, that was produced by bath application of xanthin and xanthin oxidase, did not affect, but an intracellular oxidative stress, that was produced by intracellular application of NADH and NADPH via recording pipette, decreased potassium currents. In addition, intracellular oxidative stress of single cells as measured with H2DCDA was enhanced in SHR than in WKY. Finally, mRNA expression of component of NAD(P)H oxidase such as NOX4, NOX1, and p22 was enhanced in SHR. These results suggest that decreased expression of potassium channels, and enhanced production of oxidative stress may decrease the potassium currents in SHR.
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Research Products
(11 results)
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[Journal Article] Autologous peripheral blood mononuclear cell implantation for patients with peripheral arterial disease improves limb ischemia2005
Author(s)
Ishida A, Ohya Y, Sakuda H, Ohshiro K, Higashiuesato Y, Nakaema M, Matsubara S, Yakabi S, Kakihana A, Ueda M, Miyagi C, Yamane N, Koja K, Komori K, Takishita S.
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Journal Title
Circulation Journal 69
Pages: 1260-1265
Description
「研究成果報告書概要(欧文)」より
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