2004 Fiscal Year Final Research Report Summary
Identification of novel leukemia associated antigen which may useful for immunotherapy of leukemia patients
| Project/Area Number |
15590997
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Hematology
|
|---|
| Research Institution | Niigata University |
|---|
Principal Investigator |
FURUKAWA Tatsuo Niigata University, Medical and Dental Hospital, Associate professor, 医歯学総合病院, 助教授 (00272849)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NARITA Miwako Niigata University, Faculty of Medicine, Associate professor, 医学部, 助教授 (30281009)
FUSE Ichiro Niigata University, Medical and Dental Hospital, Associate professor, 医歯学総合病院, 助教授 (90242429)
|
|---|
| Project Period (FY) |
2003 – 2004
|
| Keywords | leukemia associated antigens / CTL / SEREX |
|---|
| Research Abstract |
1.Serological identification of antigens by the recombinant expression cloning (SEREX) method has successfully identified many tumor antigens that elicit humoral immune response. In the present study, we defined a novel antigen that elicited humoral immune response in patients with leukemia and that was overexpressed in leukemia cells. Apg-1 can elicit humoral immune responses in leukemia patients and might be potential novel targets for antigen-specific immunotherapy for leukemia.
2.Using in vitro transcripted mRNA electroporation gene delivery, we developed CTL line activated by the antigen loading DC. However, we could not detect any cytotoxic activity in this activated T cells for leukemic cell line.
3.Thereafter, we identified four 9-mer peptides with HLA-A24-binding motifs encoded by Apg-1 using computer calculation. Of these, one peptide developed efficiently effector cells which lysed leukemic cell line expressing both Apg-1 and HLA-A24.
Our results indicate that Apg-1 antigen may be one of leukemia associated antigens.
|
Research Products
(8 results)
