2004 Fiscal Year Final Research Report Summary
Thansfection of oncogenes into dendritic cells utilizing receptor-mediated endocytosis of liposomes
| Project/Area Number |
15591004
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
KADOWAKI Norimitsu Kyoto University, Department of Medicine, Lecturer, 医学研究科, 講師 (60324620)
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| Co-Investigator(Kenkyū-buntansha) |
MARUYAMA Kazuo Teikyo University, Department of Biopharmaceutics, Professor, 薬学部, 教授 (30130040)
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| Project Period (FY) |
2003 – 2004
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| Keywords | dendritic cells / immunotherapy / liposome |
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| Research Abstract |
Liposomes represent a promising vehicle to deliver exogenous antigens to dendritic cells (DCs) for tumor immunotherapy. Targeting exogenous antigens to Fcγ receptors on DCs has been shown to result in efficient presentation of antigen-derived peptides on MHC class I as well as class II molecules. Here we investigated whether DCs that endocytosed physicochemically optimized, antigen-containing liposomes conjugated with IgG efficiently present antigens on MHC class I and class II molecules, and consequently induce strong anti-tumor immune responses. IgG-conjugated liposomes with 200 nm in diameter without attaching polyethylene glycol were most efficiently endocytosed by DCs. Human monocyte-derived DCs that endocytosed tetanus toxoid (TT)-containing IgG-liposomes via CD32 stimulated CD4^+ T cells more strongly than DCs pulsed with TT-containing bare liposomes or with soluble TT. Immunization of mice with DCs that endocytosed ovalbumin (OVA)-containing IgG-liposomes, but not OVA-containing bare liposomes or soluble OVA, completely prevented the growth of OVA-expressing lymphoma cells. Importantly, administration of DCs that endocytosed OVA-containing IgG-liposomes to the mice with established OVA-expressing tumors strongly suppressed tumor growth. This study demonstrates an IgG-liposome with physicochemical properties suitable for delivering antigens to DCs, and paves the way to the application of IgG-liposomes for tumor immunotherapy using DCs.
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[Journal Article] Dendritic cells that endocytosed antigen-containing IgG-liposomes elicit effective anti-tumor immunity2006
Author(s)
Kazuko Kawamura, Norimitsu Kadowaki, Ryo Suzuki, Satoshi Udagawa, Satoshi Kasaoka, Naoki Utoguchi, Toshio Kitawaki, Nakaba Sugimoto, Naoki Okada, Kazuo Maruyama, Takashi Uchiyama
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Journal Title
Journal of Immunotherapy 29(2)
Pages: 165-174
Description
「研究成果報告書概要(欧文)」より
