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2004 Fiscal Year Final Research Report Summary

Anamorsin, anti-apoptotic molecule, and hematopoiesis

Research Project

Project/Area Number 15591006
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Hematology
Research InstitutionOsaka University

Principal Investigator

SHIBAYAMA Hirohiko  Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (60346202)

Co-Investigator(Kenkyū-buntansha) KANAKURA Yuzuru  Osaka University, Graduate School of Medicine, Professor, 医学系研究科, 教授 (20177489)
MATSUMURA Itaru  Osaka University, Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (00294083)
MIZUKI Masao  Osaka University, Graduate School of Medicine, Lecturer, 医学系研究科, 講師 (80283761)
Project Period (FY) 2003 – 2004
KeywordsAnamorsin / anti-apoptosis / definitive hematopoiesis / knock-out mouse / DNA microarray / Bcl-xL / Jak2
Research Abstract

Many growth factors and cytokines prevent apoptosis. Using an expression cloning method, we identified a novel antiapoptotic molecule named Anamorsin, which does not show any homology to Down apoptosis regulatory molecules such as Bcl-2 family, caspase family, or signal transduction molecules. The expression of Anamorsin was completely dependent on stimulation with growth factors, such as interleukin 3, stem cell factor, and thrombopoietin in factor-dependent hematopoietic cell lines, and forced expression of Anamorsin conferred resistance to apoptosis caused by growth factor deprivation in vitro. Furthermore, Anamorsin was found to act as an antiapoptotic molecule in vivo because Anamorsin -/- mice die in late gestation due to defective definitive hematopoiesis in the fetal liver (FL). Although the number of hematopoietic stem/progenitor cells in the FL did not decrease in these mice, myeloid, and particularly erythroid colony formation in response to cytokines, was severely disrupted. Also, Anamorsin -/- erythroid cells initiated apoptosis during terminal maturation. As for the mechanism of Anamorsin-mediated cell survival, a microarray analysis revealed that the expression of Bcl-xL and Jak2 was severely impaired in the FL of Anamorsin -/- mice. Thus, Anamorsin is considered to be a necessary molecule for hematopoiesis that mediates antiapoptotic effects of various cytokies.

  • Research Products

    (12 results)

All 2004 2003

All Journal Article (12 results)

  • [Journal Article] Identification of a cytokine-induced antiapototic molecule anamorsin essential for definitive hematopoiesis.2004

    • Author(s)
      Shibayama H, et al.
    • Journal Title

      J Exp Med 199

      Pages: 581-592

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Roles for c-Myc in self-renewal of hematopoietic stem cells.2004

    • Author(s)
      Satoh Y, et al.
    • Journal Title

      J Biol Chem 279

      Pages: 24986-24993

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] NF-kB family proteins participate in multiple steps of hematopoiesis through elimination of reactive oxygen species.2004

    • Author(s)
      Nakata S, et al.
    • Journal Title

      J Biol Chem 279

      Pages: 55578-55586

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Identification of a cytokine-induced antiapoptotic molecule anamorsin essential for definitive hematopoiesis.2004

    • Author(s)
      Shibayama, H., et al.
    • Journal Title

      J Exp Med 199

      Pages: 581-592

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Roles for c-Myc in self-renewal of hematopoietic stem cells.2004

    • Author(s)
      Satoh, Y., et al.
    • Journal Title

      J Biol Chem 279

      Pages: 24986-24993

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] NF-kB family proteins participate in multiple steps of hematopoiesis through elimination of reactive oxygen species.2004

    • Author(s)
      Nakata, S., et al.
    • Journal Title

      J Biol Chem 279

      Pages: 55578-55586

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Reciprocal inhibition between MyoD and STAT3 in the regulation of growth and differentiation of myoblasts.2003

    • Author(s)
      Kataoka Y, et al.
    • Journal Title

      J Biol Chem 278

      Pages: 44178-44187

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Necessity of tyrosine 179 and phosphatidlinositol 3'-kinase mediated signal pathway in constitutive activation and oncogenic potential of c-kit2003

    • Author(s)
      Hashimoto K, et al.
    • Journal Title

      Blood 101

      Pages: 1094-1102

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Suppression of myeloid transcription factors and induction of STAT response genes by AML-specific Flt3 mutations.2003

    • Author(s)
      Mizuki M, et al.
    • Journal Title

      Blood 101

      Pages: 3164-3173

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Reciprocal inhibition between MyoD and STAT3 in the regulation of growth and differentiation of myoblasts.2003

    • Author(s)
      Kataoka, Y., et al.
    • Journal Title

      J Biol Chem 278

      Pages: 44178-44187

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Necessity of tyrosine 719 and phosphatidylinositol 3' kinase mediated signal pathway in constitutive activation and oncogenic potential of c-kit receptor tyrosine kinase with the Asp814Val mutation.2003

    • Author(s)
      Hashimoto, K., et al.
    • Journal Title

      Blood 101

      Pages: 1094-1102

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Suppression of myeloid transcription factors and induction of STAT response genes by AML-specific Flt3 mutations.2003

    • Author(s)
      Mizuki, M., et al.
    • Journal Title

      Blood 101

      Pages: 3164-3173

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2006-07-11  

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