2004 Fiscal Year Final Research Report Summary
CELL BIOLOGIC CHARACTERISTICS OF ENDOTHELIAL CELL PRECURSORS AND CLINICAL APPLICATIONS OF THE CELLS
| Project/Area Number |
15591019
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | JICHI MEDICAL SCHOOL |
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Principal Investigator |
MUROI Kazuo JICHI MEDICAL SCHOOL, DEPARTMENT OF MEDICINE, PROFESSOR, 医学部, 教授 (50190939)
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| Project Period (FY) |
2003 – 2004
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| Keywords | ENDOTHELIAL CELL PRECURSOR / CD34 / PERIPHERAL ARTERIAL DISEASE / VEGF |
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| Research Abstract |
We examined peripheral blood endothelial precursors in patients with chronic renal failure, coronary artery disease and large vessel aneurysm using flow cytometry and cell culture techniques. The number of both CD34 positive cells and AC133 positive cells significantly decreased in patients receiving blood dialysis due to chronic renal failure, comparing with age-matched healthy volunteers. Cultured endothelial cells derived from patients receiving blood dialysis showed decrease in up-take of LDL cholesterol. Serum VEGF levels in patients receiving blood dialysis were different from those in healthy volunteers. In coronary artery disease, the multivariate analysis showed that the only factor associated with decrease in endothelial cell precursors was diabetes mellitus. The number of endothelial cell precursors and serum VEGF levels were measured following replacement to an artificial blood vessel in patients with large vessel aneurysm : endothelial cell precursor numbers increased almost in parallel with serum VEGF levels up to seven days after the operation. These results suggest that peripheral blood endothelial cell precursors numbers decrease in chronic renal failure and the mobilization of the cells is associated with serum VEGF levels.
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