2004 Fiscal Year Final Research Report Summary
A comprehensive study of the regulatory mechanisms for prognosis-related leukaemia cell surface antigen expression
| Project/Area Number |
15591040
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | National Kyushu Cancer Center, Institute for Clinical Research |
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Principal Investigator |
OKAMURA Jun National Kyushu Cancer Center, Institute for Clinical Research, Institute for Clinical Research, Director, 臨床研究部長 (40360854)
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| Co-Investigator(Kenkyū-buntansha) |
HATTORI Hiroyoshi Univ. of Occupational and Environmental Health, Dept. of Molecular Biology, JSPS Fellow, 分子生物, 日本学術振興会特別研究員
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| Project Period (FY) |
2003 – 2004
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| Keywords | cell surface antigens / leukaemia / CD7 / prognostic factors / gene expression |
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| Research Abstract |
Using a CD7-expressing human acute myeloblasticleukaemia (AML) cell line, KG-1a, and its parentalline lacking CD7 expression, KG-1, we examined the CD7 gene structure and the levels in mRNA and protein expression, by cytogenetic observation, genomic PCR-direct sequencing, quantitative RT-PCR and flowcytometry. These analyses revealed that CD7 expression in KG-1a cells is 260-times higher than in KG -1 cells and is regulated at the mRNA level. To address further the regulatory mechanisms of CD7 expression, analyses on the transcription frequency or the mRNA stability are needed.
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