Development of tumor-specific DNA vaccines eradicating metastatic lesions

2004 Fiscal Year Final Research Report Summary

• Project/Area Number: 15591042
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 膠原病・アレルギー・感染症内科学
• Research Institution: Tohoku University
• Principal Investigator: SANO Kunio Tohoku University, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (20192601)
• Project Period (FY): 2003 – 2004
• Keywords: CpG ODN / Th1 cells / antigen-presenting cells / antigen-specific / tumor-specific immunity

Research Abstract

Although melanoma mostly affects the skin, it is notorious for its propensity to easily develop metastasis. Metastatic melanoma is highly resistant to a variety of therapies. We examined the anti-metastatic potential of peritumoral monotherapy against murine cutaneous B16F10 melanoma with synthetic oligodeoxynucleotides (ODNs) containing unmethylated CpG motifs. We demonstrated that repeated peritumoral injections of CpG ODNs significantly reduced skin tumor size. Peritumoral CpG ODN-treatment of skin tumors prevented the development of pulmonary B16F10 colonies. Adoptive transfer of splenocytes obtained from CpG ODN-treated mice markedly reduced the number of previously established pulmonary colonies in recipient naive mice. T-lymphocyte depletion studies indicated that the anti-metastatic effect was dependent on both CD4^+ and CD8^+ T cells. These results suggest that CpG ODNs are promising as a preventive and therapeutic anti-metastatic measure against melanoma.

Research Products

• [Journal Article] CpG oligodeoxynucleotides as a future vaccine for allergic diseases (2005)
  • Author(s): Sano K.et al.
  • Journal Title: Allergology International 54 Pages: 17-23
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] CpG oligodeoxynucleotides as a future vaccine for allergic diseases (2005)
  • Author(s): Sano, K, H.Shirota
  • Journal Title: Allergol.Int. 54 Pages: 17-23
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Peritumoral CpG oligodeoxynucleotide treatment inhibits experimental metastasis of skin melanoma to the lung (2004)
  • Author(s): Kunitaka, N, K.Sano et al.
  • Journal Title: Journal of Invetigatory Dermatology 123 Pages: 395-402
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Peritumoral CpG oligodeoxynucleotide treatment inhibits experimental metastasis of skin melanoma to the lung (2004)
  • Author(s): Kunikata, N, K.Sano, M.Honda1, K.Ishii, J.Matsunaga, R.Okuyama, K.Takahashi, H.Watanabe, G.Tamura, H.Tagami, T.Terui
  • Journal Title: J.Invet.Dermatol. 123 Pages: 395-402
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Oligodeoxynucleotides without CpG motifs work as adjuvant for the induction of Th2 differentiation in a sequence-independent manner (2003)
  • Author(s): Sano K.et al.
  • Journal Title: Journal of Immunology 170 Pages: 2367-2373
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Oligodeoxynucleotides without CpG motifs work as adjuvant for the induction of Th2 differentiation in a sequence-independent manner. (2003)
  • Author(s): Sano, K., H.Shirota, T.Terui, T.Hattori, G.Tamura
  • Journal Title: J.Immunol. 170 Pages: 2367-2373
  • Description: 「研究成果報告書概要(欧文)」より