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2004 Fiscal Year Final Research Report Summary

Immunological Regulation of auto-reactive RP105-negative B cells

Research Project

Project/Area Number 15591057
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 膠原病・アレルギー・感染症内科学
Research InstitutionSaga University (2004)
佐賀医科大学 (2003)

Principal Investigator

KOARADA Syuichi  Saga University, Faculty of Medicine, Research Associate, 医学部, 助手 (50304887)

Co-Investigator(Kenkyū-buntansha) TADA Yoshifumi  Saga University, Faculty of Medicine, Assistant Professor, 医学部, 講師 (70284627)
NAGASAWA Kohei  Saga University, Faculty of Medicine, Professor, 医学部, 教授 (00108721)
Project Period (FY) 2003 – 2004
KeywordsRP105 / SLE / B cells / Immunological Regulation / Siogren's syndrome / dermatomyositis / Toll-like receptor / atuoimmune dieseases
Research Abstract

The function and expression of RP105 on B cells in autoimmune diseases have not been fully clarified, especially in human. To investigate the RP105 expression of autoantibody producing B cell subsets in patients with Systemic Lupus Erythematosus(SLE), we performed phenotypic and cell viability study for RP105-negative B cells in SLE. Expression of B cell markers on 105-negative and RP105-positive B cells was analyzed by a flow cytometry. The peripheral blood mononuclear cells were cultured with various stimulators with or without their blockers and were analyzed for expression RP105 on B cells. RP105-negative B cells form SLE showed increased expression of surface levels of CD38 and CD27 was found in RP105-negative B cells compared with RP105-positive B cells from SLE. In the culture with activated T cell and BAFF/APRIL, increased survival of RP105-negative B cells were found in SLE. In SLE, B cells, differntially express various markers of B cells and the survival was maintained by T cell stimulation and BAFF/APRIL stimulation. RP105-negative B cells may be targets for the treatment of SLE.

  • Research Products

    (14 results)

All 2005 2004 2003

All Journal Article (13 results) Book (1 results)

  • [Journal Article] Two cases of antinuclear antibody negative lupus showing increased proportion of RP105-lacking B cells.2005

    • Author(s)
      Koarada S, Ide M, Haruta Y, Tada Y, Ushiyama O, Morito F, Ohta A
    • Journal Title

      J Rheumtol. 32

      Pages: 562-564

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Two cases of antinuclear antibody negative lupus showing increased proportion of B cells lacking RP105.2005

    • Author(s)
      Koarada S, Ide M, Haruta Y, Tada Y, Ushiyama O, et al.
    • Journal Title

      J Rheumatol. 32(3)

      Pages: 562-564

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Increased entry of CD4+ T cells into the Th1 cytokine effector pathway during T-cell division following stimulation in Behcet's dise2004

    • Author(s)
      Koarada S, Haruta Y, Tada Y, Ushiyama O, Morito F, Ohta A
    • Journal Title

      heumatology(Oxford). 43

      Pages: 843-851

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Genetic control of autoimmunity : protection from diabetes, but spontaneous autoimmune biliary disease in a nonobese diabetic2004

    • Author(s)
      Koarada S, Wu Y, Fertig N, Sass DA, Nalesnik M, Todd JA, Lyons PA
    • Journal Title

      J Immunol. 173

      Pages: 2315-2323

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Nonobese diabetic CD4 lymphocytosis maps outside the MHC locus on chromosome 17.2004

    • Author(s)
      Koarada S, Wu Y, Yim YS, Wakeland EW, Ridgway WM.
    • Journal Title

      Immunogenetics. 56

      Pages: 333-337

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Nonobese diabetic CD4 lymphocytosis maps outside the MHC locus on chromosome 17.2004

    • Author(s)
      Koarada S, Wu Y, Yim YS, Wakeland EW, Ridgway WM.
    • Journal Title

      Immunogenetics. 56(5)

      Pages: 333-337

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Genetic control of autoimmunity : protection from diabetes, but spontaneous autoimmune biliary disease in a nonobese diabetic congenie strain.2004

    • Author(s)
      Koarada S, Wu Y, Fertig N, Sass DA, Nalesnik M, et al.
    • Journal Title

      J Immunol. 15;173(4)

      Pages: 2315-2323

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Increased entry of CD4+ T cells into the Th1 cytokine effector pathway during T-cell division following stimulation in Behcet's disease.2004

    • Author(s)
      Koarada S, Haruta Y, Tada Y, Ushiyama O, Morito F, et al.
    • Journal Title

      Rheumatology. 43(7)

      Pages: 843-851

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Acceleration of the onset of collagen-induced arthritis by a deficiency of platelet endothelial cell adhesion molecule 1.2003

    • Author(s)
      Tada Y, Koarada, S, Morito F, Ushiyama O, Haruta Y, Kanegae F
    • Journal Title

      Arthritis Rheum. 48

      Pages: 3280-3290

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Retinal findings in systemic sclerosis : a comparison with nailfold capillaroscopic patterns.2003

    • Author(s)
      Ushiyama O, Ushiyama K, Yamada T, Koarada S, Tada Y, Suzuki N
    • Journal Title

      Ann Rheum Dis. 62

      Pages: 204-207

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Acceleration of the onset of collagen-induced arthritis by a deficiency of platelet endothelial cell adhesion molecule 1.2003

    • Author(s)
      Tada Y, Koarada S, Morito F, Ushiyama O, Haruta Y, et al.
    • Journal Title

      Arthritis Rheum. 48(11)

      Pages: 3280-3290

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Frequency and analysis of factors closely associated with the development of depressive symptoms in patients with scleroderma.2003

    • Author(s)
      Matsuura E, Ohta A, Kanegae F, Haruda Y, Ushiyama O, Koarada S, et al.
    • Journal Title

      J Rheumatol. 30(8)

      Pages: 1782-1787

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Retinal findings in systemic sclerosis : a comparison with nailfold capillaroscopic patterns.2003

    • Author(s)
      Ushiyama O, Ushiyama K, Yamada T, Koarada S, Tada Y, et al.
    • Journal Title

      Ann Rheum Dis. 62(3)

      Pages: 204-207

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] 自然免疫と疾患2004

    • Author(s)
      小荒田秀一, 長澤浩平
    • Total Pages
      124
    • Publisher
      医歯薬出版
    • Description
      「研究成果報告書概要(和文)」より

URL: 

Published: 2006-07-11  

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