2004 Fiscal Year Final Research Report Summary
Immunological Regulation of auto-reactive RP105-negative B cells
| Project/Area Number |
15591057
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
膠原病・アレルギー・感染症内科学
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| Research Institution | Saga University (2004)
佐賀医科大学 (2003) |
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Principal Investigator |
KOARADA Syuichi Saga University, Faculty of Medicine, Research Associate, 医学部, 助手 (50304887)
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| Co-Investigator(Kenkyū-buntansha) |
TADA Yoshifumi Saga University, Faculty of Medicine, Assistant Professor, 医学部, 講師 (70284627)
NAGASAWA Kohei Saga University, Faculty of Medicine, Professor, 医学部, 教授 (00108721)
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| Project Period (FY) |
2003 – 2004
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| Keywords | RP105 / SLE / B cells / Immunological Regulation / Siogren's syndrome / dermatomyositis / Toll-like receptor / atuoimmune dieseases |
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| Research Abstract |
The function and expression of RP105 on B cells in autoimmune diseases have not been fully clarified, especially in human. To investigate the RP105 expression of autoantibody producing B cell subsets in patients with Systemic Lupus Erythematosus(SLE), we performed phenotypic and cell viability study for RP105-negative B cells in SLE. Expression of B cell markers on 105-negative and RP105-positive B cells was analyzed by a flow cytometry. The peripheral blood mononuclear cells were cultured with various stimulators with or without their blockers and were analyzed for expression RP105 on B cells. RP105-negative B cells form SLE showed increased expression of surface levels of CD38 and CD27 was found in RP105-negative B cells compared with RP105-positive B cells from SLE. In the culture with activated T cell and BAFF/APRIL, increased survival of RP105-negative B cells were found in SLE. In SLE, B cells, differntially express various markers of B cells and the survival was maintained by T cell stimulation and BAFF/APRIL stimulation. RP105-negative B cells may be targets for the treatment of SLE.
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[Book] 自然免疫と疾患2004
Author(s)
小荒田秀一, 長澤浩平
Total Pages
124
Publisher
医歯薬出版
Description
「研究成果報告書概要(和文)」より
