Therapeutic effect of hematopoietic stem cell transplantation in combination with tissue regenerative factor on systemic sclerosis

2004 Fiscal Year Final Research Report Summary

• Project/Area Number: 15591071
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 膠原病・アレルギー・感染症内科学
• Research Institution: Hyogo College of Medicine
• Principal Investigator: IWASAKI Tsuyoshi Hyogo College of Medicine, Faculty of Medicine, Associate Professor, 医学部, 助教授 (10151721)
• Co-Investigator(Kenkyū-buntansha): FUJIMOTO Jiro Hyogo College of Medicine, Faculty of Medicine, Professor, 医学部, 教授 (90199373) ; SANO Hajime Hyogo College of Medicine, Faculty of Medicine, Professor, 医学部, 教授 (00196304)
• Project Period (FY): 2003 – 2004
• Keywords: systemic sclerosis / HGF / hematopoietc stem cell transplantation / gene therapy / HVJ-liposome

Research Abstract

1)Transplantation of T-cell depleted bone marrow cells from BALB/c mice improved skin thickness by Tsk mice
2)HGF gene transfection improved skin thickness by Tsk mice.
3)Combination of bone marrow transplantation and HGF gene therapy significantly improved skin thickness by Tsk mice but did not improved pulmonary fibrosis.
4)Transplantation of T-cell depleted bone marrow cells shifted immune responses from Th2 to Th1.
5)Donor cells were engrafted at 2 weeks after transplantation, but were rejected at 4 weeks after transplantation.