2004 Fiscal Year Final Research Report Summary
The possibility to the cell therapy of the stem cell which has the plasticity which exists in the human umbilical cord blood
| Project/Area Number |
15591088
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Tokyo Women's Medical University (2004)
The University of Tokyo (2003) |
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Principal Investigator |
TSURUTA Toshihisa Tokyo Women's Medical University, Faculty of Medicine, Lecturer, 医学部, 講師 (70197771)
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| Co-Investigator(Kenkyū-buntansha) |
TSUJI Kohichiro University of Tokyo, Institution of Science, Assistant Professor, 医科学研究所, 助教授 (50179991)
TOJO Arinobu University of Tokyo, Institution of Science, Professor, 医科学研究所, 教授 (00211681)
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| Project Period (FY) |
2003 – 2004
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| Keywords | umbilical cord blood / stem cell / CD34 / hemangioblast / endotherial cell / TEK / estrogen receptor / cord blood transplantation |
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| Research Abstract |
We paid attention to TEK and CD34 expressed to both of the blood cell and the vascular endothelial cell to examine the plasticity of the umbilical cord blood first. Restructuring of the human hematopoiesis was recognized in the all cases when 1x 10^4 CD34+TEK+ cells and CD34+TEK- cells which it could get from human umbilical cord blood, were transplanted to each 7 ischemic mouse models. Furthermore, though differentiation to the endothelial cells were confirmed with CD34+TEK+ cells transplantation mouse by the all cases, a differentiation to the endothelial cells that only three cases were restructured of the human hematopoiesis in the high rate was recognized with a CD34+TEK-cell transplantation mouse. It could think that it had differentiation potency to the endothelial cells that CD34+TEK+ cells are higher than CD34+TEK- cells, and the possibility that undifferentiated precursor cell, that is, hemangioblast existed was suggested by the inside of the umbilical cord blood more than TE
… More
K+ endothelial precursor cell. The analysis of the gene expression which controls the differentiation, proliferation of the umbilical cord blood subsequently was done. As for the hematopoiesis restructuring pattern after the umbilical cord blood transplantation, it knows that it is different from the thing after the bone marrow transplantation. ERα mRNA was expressed in the leukemia cell line of most B-cell lineage, and ERβ mRNA got the result that the Daudi cell was strongly expressed only in the lymphoid cell with the maturity tendency. And, both expressions were recognized as a in the predominance with umbilical cord blood mononuclear in the low quantity of a measurement limit. Furthermore, we did the co-culture experiment of the umbilical cord blood and the mouse stromal cell (HESS-V) to establish the line to cultivate stem cell under the serum-free place. As for the umbilical cord blood CD34 positive cell, it found that it could be cultivated, it was maintained without differentiation, by using HESS-V to the approx. 3 weeks. At present, use this experimental line, and do a change in nature of the steroid and the umbilical cord blood mononuclear by the addition such as a hematopoietic growth factor, an ER congenic experiment, and so on, and the examination of the potential of the umbilical cord blood is being done. Less
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Research Products
(42 results)
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[Journal Article] Single-institute comparative analysis of unrelated bone marrow transplantation and cord blood transplantation for adult patients with hematological malignancies.2004
Author(s)
Takahashi S.Iseki T, Ooi J, Tomonari A, Takasugi K, Shimohakamada Y, Yamada T, Uchimaru K, Tojo A, Shirafuji N, Kodo H, Tani K, Takahashi T, Yamaguchi T, Asano S.
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Journal Title
Blood 104
Pages: 3813-3820
Description
「研究成果報告書概要(和文)」より
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[Journal Article] A novel maxizyme vector targeting a bcr-abl fusion gene induced specific cell death in Philadelphia chromosome-positive acute lymphoblastic leukemia.2004
Author(s)
Soda Y, Tani K, Bai Y, Saiki M, Chen M, Izawa K, Kobayashi S, Takahashi S, Uchimaru K, Kuwabara T, Warashina M, Tanabe T, Miyoshi H, Sugita K, Nakazawa S, Tojo A, Taira K, Asano S
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Journal Title
Description
「研究成果報告書概要(和文)」より
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[Journal Article] CD19-targeting liposomes containing imatinib efficiently kill Philadelphia chromosome-positive acute lymphoblastic leukemia cells.2004
Author(s)
Harata M, Soda Y, Tani K, Ooi J, Takizawa T, Chen M, Bai Y, Izawa K, Kobayashi S, Tomonari A, Nagamura F, Takahashi S, Uchimaru K, Iseki T, Tsuji T, Takahashi TA, Sugita K, Nakazawa S, Tojo A, Maruyama K, Asano S
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Journal Title
Blood 104
Pages: 1442-1449
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Hematopoietic ativity of common marmoset CD34^+ cells by a novel monoclonal antibody MA24.2004
Author(s)
Izawa K, Tani K, Nakazaki Y, Hibino H, Sugiyama H, Kawasaki A, Sasaki E, Nishioka C, Ishii H, Soda Y, Yagita H, Tanioka Y, Tojo A, Asano S
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Journal Title
Brit J Haematol 126
Pages: 711-714
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Unrelated cord blood transplantation after myeloablative conditioning in patients over age of 45 years.2004
Author(s)
Ooi J, Iseki T, Takahashi S, Tomonari A, Takasugi k, Uchiyama y, Konuma T, Hutami T, Nomura A, Nakayama S, Soda Y, Ohno N, Nagamura F, uchimaru K, Tojo A, Tani K, Asanao S
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Journal Title
Brit J haematol 126
Pages: 711-714
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Single-institute comparative analysis of unrelated bone marrow transplantation and cord blood transplantation for adult patients with hematological malignancies.2004
Author(s)
Takahashi S, Iseki T, Ooi J, Tomonari A, Takasugi K, Shimohakamada Y, Yamada T, Uchimaru K, Tojo A. Shirafuji N, Kodo H, Tani K, Takahashi T, Yamaguchi T, Asano S.
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Journal Title
Blood 104
Pages: 3813-3820
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Unrelated cord blood transplantation with reduced-intensity conditioning following autologous transplantation for multiple myeloma.2004
Author(s)
Yamada T, Tomonari A, Takahashi S, Ooi J, Iseki T, Shimohakamada Y, Takasugi K, Ohno N, Nagamura F, Uchimaru K, Tojo A, Asano S
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Journal Title
Int J Hematol 80
Pages: 377-380
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] MxA expression in patients with viral infection after allogeneic stem cell transplantation.2003
Author(s)
Yoshimatsu T, Manabe A, Ebihara Y, Tanaka R, Ooi J, Iseki T, Shirafuji N, Maekawa T, Asano S, Yoshikawa N, Tsuii K
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Journal Title
Bone Marrow Transplant 32
Pages: 313-316
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Mammalian twisted gastrulation is essential for skeleto-lymphogenesis.2003
Author(s)
Nosaka T, Morita S, Kitamura H, Nakajima H, Shibata F, Kataoka Y, Morikawa Y, Kawashima T, Ito T, Ebihara Y, Senba E, Tsuji K, Makishita F, Yoshida N, Kitamura T
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Journal Title
Mol Cell Biol 23
Pages: 2969-2980
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Herpes simplex virus infection in adult patients after unrelated cord blood transplantation : a single institute experience in Japan.2003
Author(s)
Tomonari A, Takahashi S, Iseki T, Ooi J, Yamada T, Takasugi K, Shimohakamada Y, Ohno N, Nagamura F, Uchimaru K, tani K, Tojo A
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Journal Title
Bone Marrow Transplant 33
Pages: 317-320
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Ex vivo manipulation of umbilical cord- derived hematopoietic stem/progenit or cells with recombinant human stem cell factor can up-regulate levels of homing-essential molecules to increase their transmigratory potential.2003
Author(s)
Zheng Y, Watanabe N, nagamura-Inoue T, Igura K, Nagayama H, Tojo A, Tanosaki R, Takaue Y, Okamoto S, Takahashi T, Asanao S
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Journal Title
Exp Hematol 31
Pages: 1237-1246
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Unrelated cord blood transplantation for adult patients with advanced myelodysplastic syndrome.2003
Author(s)
Ooi J, Iseki T, Takahashi S, Tomonari A, Ishii K, Takasugi K, Shimohakamada Y, Ohono N, Uchimaru K, Nagamura F, Tojo A, Asano S
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Journal Title
Blood 101
Pages: 4711-4713
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Varicella-zoster virus infection in adult patients after unrelated cord blood transplantation : a single institute experience in Japan.2003
Author(s)
Tomonari A, Iseki T, Takahashi S, Ohi J, Tkasugi K, Shimohakamada Y, Ohno N, Nagamura F, Uchimarn K, Tani K, Tojo A, Asano S
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Journal Title
Br j Haematol 122
Pages: 802-805
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Herpes simplex virus infection in adult patients after unrelated cord blood transplantation : a single institute experience in Japan.2003
Author(s)
Tomonari A, Takahashi S, Iseki T, Ooi J, Yamada T, Takasugi K, Shimohakamada Y, Ohno N, Nagamura F, Uchimaru K, Tani K, Tojo A, Asano S
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Journal Title
Bone Marrow Transplant 33
Pages: 317-320
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Unrelated cord blood transplantation for adult patients with advanced myelodysplastic syndrome.2003
Author(s)
Ooi J, Iseki T, Takahashi S, Tomonari A, Ishii K, Takasugi K, Shimohakamada Y, Ohno N, Uchimaru K, Nagamura F, Tojo A, Asano S
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Journal Title
Blood 101
Pages: 4711-4713
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Methylation status of the p15 and p16 genes in paediatric myelodysplastic syndorome and juvenile myelomonocytic leukemia.
Author(s)
Hasegawa D, Manabe A, Kubota T, Kawasaki H, Hirose I, Ohtsuka Y, Tsuruta T, Ebihara Y, Goto Y, Zhao XY, Sakashita K, Koike K, Isomura M, Kojima S, Hoshika A, Tsuji K
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Journal Title
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Unrelated cord blood transplantation with reduced-intensity conditioning following autologous transplantation for multiple myeloma.
Author(s)
Yamada T, Tomonari A, Takahashi S, Ooi J, Iseki T, Shimohakamada Y, Takasugi K, Ohno N, Nagamura F, Uchimaru K, Tojo A, Asano S
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Journal Title
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Anti-NK cell treatment induces stable mixed chimerism in MHC-mismatched, T-cell depleted, nonmyeloablative bone marrow transplantation.
Author(s)
Cho SG, Shuto Y, Soda Y, Nakazaki Y, Izawa K, Uchimaru K, Takahashi S, Tani K, Tojo A, Asano S
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Journal Title
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Methylation status of the p15 and p16 genes in paediatric myelodysplastic syndrome and juvenile myelomonocytic leukaemia.
Author(s)
Hasegawa D, Manabe A, Kubota T, Kawasaki H, Hirose I, Ohtsuka Y, Tsuruta T, Ebihara Y, Goto Y, Zhao XY, Sakashita K, Koike K, Isomura M, Kojima S, Hoshika A, Tsuji K, Nakahata T
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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