2004 Fiscal Year Final Research Report Summary
Renoprotective role of interstitial macrophages in the evolution of renal fibrosis
| Project/Area Number |
15591124
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
NISHIDA Masashi Kyoto Prefectural University of Medicine, Department of Pediatric Cardiology and Nephrology, Assistant Professor, 医学研究科, 助手 (50275202)
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| Co-Investigator(Kenkyū-buntansha) |
HAMAOKA Kenji Kyoto Prefectural University of Medicine, Department of Pediatric Cardiology and Nephrology, Professor, 医学研究科, 教授 (60189602)
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| Project Period (FY) |
2003 – 2004
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| Keywords | renal fibrosis / macrophage / unilateral ureteral obstruction |
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| Research Abstract |
We performed adoptive transfer of bone marrow-derived(BM) macrophages following pharmacological depletion of leukocytes in a mouse model of unilateral ureteral obstruction(UUO), a well-established experimental model of progressive renal interstitial fibrosis. Treatment with cyclophosphamide(CPM) caused marked decrease in the numbers of F4/80-positive interstitial macrophages as well as in peripheral blood leukocyte counts, and adoptive transfer of BM macrophages to CPM-treated mice resulted in significant increase in the numbers of interstitial macrophages both at day 5 and at day 14 after UUO. At day 5 after UUO, no significant change was observed in the degree of renal interstitial fibrosis either by treatment with CPM or with CPM+macrophage. However, at day 14 after UUO, treatment with CPM caused significant increase in the degree of interstitial fibrosis, and adoptive macrophage transfer to these mice attenuated this enhancement in renal fibrosis. Our result suggests the role of infiltrating macrophages on facilitating tissue repair at late stage of UUO.
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