2005 Fiscal Year Final Research Report Summary
Analysis of host factors on neurotropism of SSPE virus
| Project/Area Number |
15591126
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Osaka City University |
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Principal Investigator |
MATSUOKA Osamu Osaka City University, Graduate School of Medicine, Lecturer, 大学院・医学研究科, 講師 (20117972)
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| Co-Investigator(Kenkyū-buntansha) |
YAMANO Tunekazu Osaka City University, Graduate School of Medicine, Professor, 大学院・医学研究科, 教授 (20093172)
HATTORI Hideji Osaka City University, Graduate School of Medicine, Lecturer, 大学院・医学研究科, 講師 (70244639)
OGURA Hisashi Osaka City University, Graduate School of Medicine, Professor, 大学院・医学研究科, 教授 (10115222)
AYATA Minoru Osaka City University, Graduate School of Medicine, Research, 大学院・医学研究科, 助手 (90222702)
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| Project Period (FY) |
2003 – 2005
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| Keywords | Subacute Sclerosing Panencephalitis / SSPE virus / measles virus / cell tropism / SLAM (CD150) / CD46 |
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| Research Abstract |
Our results obtained so far suggested a possibility of the 3rd receptor for SSPE strains different from CD150 (SLAM) and CD46. The purpose of this project is to further confirm existence of this novel receptor and to clone cDNA of this molecule.
To confirm its existence, virus entry assay was performed using VSV pseudotype system together with entry blocking by the specific monoclonal antibody against CD150 or CD46. SSPE strains were found to use CD150 but not CD46, in addition, a certain cell surface molecule except for the both ones. This novel molecule was expressed human neuroblastoma cells and human glioma cells. As another estimation on virus entry, syncytium formation by SSPE psedovirions prepared by treatment of the infected cells with cytochalasin D was monitored and the same results as VSV pseudotype system were also obtained.
Based on the above confirmation, cDNA cloning of the 3rd receptor was tried using ecotropic retroviral vector from cDNA library of Vero cells, which seemed to express the molecule most among our available cells examined. In addition to it, preparation of mouse monoclonal antibody blocking entry of SSPE strains into Vero cells was tried to catch the novel molecule. At present, we have not yet succeeded probably because of too little amount of its expression. These are now still in progress. As a different approach, some more sensitive and effective cloning methods are under consideration.
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Research Products
(32 results)
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[Journal Article] Probe typing of Noroviruses detected in Osaka City, Japan2005
Author(s)
Iritani, N., Seto, Y., Kubo, H., Haruki, K., Murakami, T., Kaida, A., Ayata, M., Ogura, H.
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Journal Title
Jpn.J.Infect.Dis. 28
Pages: 53-54
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Genotyping of Norovirus strains detected in outbreaks between April 2002 and March 2003 in Osaka City, Japan2005
Author(s)
Seto, Y., Iritani, N., Kubo, H., Kaida, A., Murakami, T., Haruki, K., Nishio, O., Ayata, M., Ogura, H.
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Journal Title
Microbiol.Immunol. 49
Pages: 275-283
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Wild-type measles virus infection in human CD46/CD150-transgenic mice : CD11c-positive dendritic cells establish systemic viral infection.2005
Author(s)
Shingai, M., Inoue, N., Okuno, T., Okabe, M., Akazawa, T., Miyamoto, Y., Ayata, M., Honda, K., Kurita-Taniguchi, M., Matsumoto, M., Ogura, H., Taniguchi, T., Seya, T.
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Journal Title
J.Immunol. 175
Pages: 3252-3261
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] An infant with idiopathic orbital myositis poorly responsive to steroid therapy : a case report.2005
Author(s)
Hattori, H., Ohnishi, S., Nakagawa, Y., Ikemiya, M., Yamato, K., Matsuoka, O., Yokoyama, T., Yamano, T.
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Journal Title
Brain Dev. 27
Pages: 160-162
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Efficacy of Intravenous Lidocaine Therapy in the Management of Status Epileptics in Childhood.2005
Author(s)
Hattori, H., Yamano, T., Hayashi, K., Osawa, M., Hamano, S., Kaneko, K.
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Journal Title
Epilepsia 46(Suppl.2)
Pages: 4
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Clinical features of patients with infantile epilepsy and agenesis of corpus callpsum.2005
Author(s)
Ishida, H., Hattori, H., Tanaka, K., Otani, S., Matsuoka, O., Yamano, T.
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Journal Title
Epilepsia 46(Suppl.3)
Pages: 14
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Infection of different cell lines of neural origin with subacute sclerosing panencephalitis (SSPE) virus2004
Author(s)
Ishida, H., Ayata, M., Shingai, M., Katayama, Y., Matsunaga, I., Seya, T., Yanagi, Y., Yamano, T., Ogura, H.
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Journal Title
Microbiol.Immunol. 48
Pages: 277-287
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Behavioral Teratology Committee of the Japanese Teratology Society. Comments from the Behavioral Teratology Committee of the Japanese Teratology Society on OECD guideline for the testing of chemicals, proposal for a new guideline 426, developmental neurotoxicity study, draft document (September 2003).2004
Author(s)
Fukui Y, Ema M, Fujiwara M, Higuchi H, Inouye M, Iwase T, Kihara T, Nishimura T, Oi A, Ooshima Y, Otani H, Shinomiya M, Sugioka K, Yamano T, Yamashita KH, Tanimura T.
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Journal Title
Congenit Anom. 44(3)
Pages: 172-177
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Substrate Recognition and molecular mechanism of fatty acid hydroxylation by cytochrome P450 from Bacillus subtilis2003
Author(s)
Lee, D-S., Yamada, A., Sugimoto, H., Matsunaga, I., Ogura, H., Ichihara, K., Adachi, S., Park, S-Y., Shiro, Y.
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Journal Title
J.Biol.Chem. 278
Pages: 9761-9767
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Prevalence of Norwalk virus infections in viral gastroenteritis among children in Osaka City, Japan2003
Author(s)
Iritani, N., Seto, Y., Kubo, H., Murakami, T., Haruki, K., Ayata, M., Ogura, H.
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Journal Title
J.Clin.Microbiol. 41(4)
Pages: 1756-1759
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Receptor use by vesicular stomatitis virus pseudotypes with glycoproteins of defective variants of measles virus isolated from brains of patients with subacute sclerosing panencephalitis2003
Author(s)
Shingai, M., Ayata, M., Ishida, H., Matsunaga, I., Katayama, Y., Seya, T., Tatsuo, H., Yanagi, Y., Ogura, H.
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Journal Title
J.Gen.Virol. 84
Pages: 2133-2143
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Mechanism of up-regulation of human Toll-like receptor (TLR) 3 secondary to infection of measles virus attenuated strains2003
Author(s)
Tanabe, M., Kurita-Taniguchi, M., Takeuch, K., Takeda, M., Ayata, M., Ogura, H., Matsumoto, M., Seya, T.
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Journal Title
Biochem.Bioph.Res.Co. 311
Pages: 39-48
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Interictal patterns of cerebral glucose metabolism, perfusion, and magnetic field in mesial temporal lobe epilepsy.2003
Author(s)
Sakamoto S, Tsuyuguchi N, Takami T, Morino M, Goto T, Hattori H, Tsutada T, Haque M, Sunada I, Shimogawara M, Hara M.
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Journal Title
Epilepsia Sep 44(9)
Pages: 1196-1206
Description
「研究成果報告書概要(欧文)」より
