2004 Fiscal Year Final Research Report Summary
Does the muation of epiplakin gene induce a disease? - Elimination of epiplakin by gene targeting
| Project/Area Number |
15591184
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Oita University (2004)
大分医科大学 (2003) |
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Principal Investigator |
FUJIWARA Sakuhei Oita University, Faculty of Medicine, Professor, 医学部, 教授 (90181411)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIOKA Hidekatsu Oita University, Faculty of Medicine, Professor, 医学部, 教授 (00222430)
SUMIYOSHI Hideaki Oita University, Faculty of Medicine, Assistant, 医学部, 助手 (60343357)
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| Project Period (FY) |
2003 – 2004
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| Keywords | Bullous Pemphigoid / plectin / autoantigen / knock out mouse / keratinocyte / intermediate filament / cell attachment / epiplakin |
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| Research Abstract |
Abstract
Epiplakin(EPPK) was originally identified as a human epidermal autoantigen. To identify the function of epiplakin, we generated epiplakin "knock-out" mice. These mice developed normally, with apparently normal epidermis and hair. Electron microscopy after immunostaining revealed the presence of EPPK adjacent to keratin filaments in wild-type mice, suggesting that epiplakin might associate with keratin. The appearance and localization of keratin bundles in intact epidermal keratinocytes of EPPK^<-/-> mice were similar to those in wild-type mice. Wounds on backs of EPPK^<-/-> mice closed more rapidly than those on backs of wild-type and heterozygous mice. Outgrowth of keratinocytes from skin explants from knock-out mice was enhanced as compared to outgrowth from explants from wild-type mice even in the presence of mitomycin C, suggesting that the difference in keratinocyte outgrowth might be due to a difference in the speed of migration of keratinocytes. At wound edges in wild-type mice, EPPK was expressed in proliferating keratinocytes in conjunction with keratin 6. In EPPK^<-/-> mice, no similar proliferating keratinocytes were observed but migrating keratinocytes weakly expressed keratin 6. EPPK was coexpressed with keratin 6 in some keratinocytes in explant cultures from wild mice. We propose that EPPK might be linked functionally with keratin 6.
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[Journal Article] Epiplakin, a novel member of the plakin family originally identified as a 450-kDa human epidermal autoantigen : Structure and tissue localization.2001
Author(s)
Fujiwara.S, Takeo.N, Otani.Y, Parry.DAD, Kunimatsu.M, Lu.R, Sasaki.M, Matsuo.N, Khaleduzzaman.M, Yoshioka.H
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Journal Title
J Biol Chem 276
Pages: 13340-13347
Description
「研究成果報告書概要(欧文)」より
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