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2004 Fiscal Year Final Research Report Summary

Promoter analysis of the interferon regulatory factor 2 and the SNP analysis of the gene

Research Project

Project/Area Number 15591187
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionOsaka City University Graduate School of Medicine

Principal Investigator

FUKAI Kazuyoshi  Osaka City University, Department of Dermatology, Associate professor, 大学院・医学研究科, 助教授 (20244642)

Project Period (FY) 2003 – 2004
KeywordsIRF2 / polymorphism / promoter / psoriasis / 多因子遺伝
Research Abstract

Interferon regulatory factor 2 (IRF-2) is a transcriptional regulatory protein which represses the expression of interferon-alpha/beta genes. In mice lacking IRF-2, the inflammatory skin disease very similar to human psoriasis develops spontaneously. In addition, IRF-2 gene is located at human chromosome 4q35, where a familial psoriasis susceptibility locus has been mapped. Therefore, we hypothesized the IRF-2 gene is a possible candidate gene for psoriasis.
Genotyping of -535G>A was performed by NlaIII-restriction flagment polymorphism (RFLP). Gel-shift and super-shift analysis were employed for the accessment of the binding abilities of transcription factors. To demonstrate differential transcriptional activities, luciferase reporter assays were perforemed.
We identified a -535G>A polymorphism in the IRF-2 transcriptional promoter. The -535A allele was more frequent (p= 0.035) in early-onset (less than 41 years of age) psoriasis in Japan, although this was not associated with the old-onset (41 years or older) psoriasis. The -535G allele was found to bind upstream stimulatory factor (USF) by the supershift analysis, whereas the -535A allele did not bind USF. However, the reporter assay revealed no statistical difference of the transcriptional activity between -535A allele and -535G allele. Furthermore, co-transfection of the USF-1 expression vector did not stimulate the promoter activity of our luciferase reporter system.

  • Research Products

    (10 results)

All 2005 2004 2003 Other

All Journal Article (10 results)

  • [Journal Article] Analysis of KIT, SCF and initial screening of SLUG genes in patients with piebaldism.2005

    • Author(s)
      Murakami T et al.
    • Journal Title

      J Invest Dermatol 124

      Pages: 670-672

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Polymorphisms in the promoter region of the IL-4R are associated with atopic dermatitis in Japan.2004

    • Author(s)
      Hosomi N, Fukai K et al.
    • Journal Title

      J Invest Dermatol 122

      Pages: 843-845

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Proximal promoter polymorphisms of the IL-4R alpha are associated with psortasis : inverse association pattern compared with atopic dermatitis.2004

    • Author(s)
      Fukai K, Hosomi N et al.
    • Journal Title

      J Dermatol Sci 35

      Pages: 78-80

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Novel KIT mutations in patients with piebaldism.2004

    • Author(s)
      Murakami T et al.
    • Journal Title

      J Dermatol Sci 35

      Pages: 29-33

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Proximal promoter polymorphisms of the IL4R alpha are associated with psoriasis : inverse association pattern compared with atopic dermatitis.2004

    • Author(s)
      Fukai K, Hosomi N et al.
    • Journal Title

      J Dermatol Sci 35

      Pages: 78-80

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Angelman syndrome associated with OLAZ due to intragenic deletion of the P gene2003

    • Author(s)
      Fridman C, Hosomi N et al.
    • Journal Title

      Am J Med Genet 119

      Pages: 180-183

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Association of SPINK5 gene polymorphisms with atopic dermatitis in the Japanese population2003

    • Author(s)
      Kato A, Fukai K et al.
    • Journal Title

      Br J Dermatol 148

      Pages: 665-669

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Angelman syndrome associated with OCA2 due to intragenic deletion of the P gene.2003

    • Author(s)
      Fridman C, Hosomi N et al.
    • Journal Title

      Am J Med Genet 119

      Pages: 180-183

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Association of SPINK5 gene polymorphisms with atopic dermatitis in the Japanese population.2003

    • Author(s)
      Kato A, Fukai K et al.
    • Journal Title

      Br J Dermatol 148

      Pages: 665-669

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Analysis of KIT, SCF and initial screening of SLUG genes in patients with piebaldism

    • Author(s)
      Murakami T et al.
    • Journal Title

      J Invest Dermatol (印刷中)

    • Description
      「研究成果報告書概要(和文)」より

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Published: 2007-12-13  

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