2004 Fiscal Year Final Research Report Summary
Biological modulation of steroid for bile output
| Project/Area Number |
15591338
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Nagoya University |
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Principal Investigator |
YUASA Norihiro Nagoya University, University Hospital, Assistant Professor, 医学部附属病院, 講師 (00303610)
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| Co-Investigator(Kenkyū-buntansha) |
NIMURA Yuji Nagoya University, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (80126888)
NAGINO Masato Nagoya University, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (20237564)
ODA Koji Nagoya University, University Hospital, Research Associate, 医学部附属病院, 助手 (30311715)
ARAI Toshiyuki Nagoya University, University Hospital, Research Associate, 医学部附属病院, 助手 (80335041)
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| Project Period (FY) |
2003 – 2004
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| Keywords | steroid / basal bile output / hydrogen sulfide / cytathionine γ-lyase / cysteine / propagylglycine / NaHS / biabonate |
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| Research Abstract |
1.Peritoneal administration of steroid, 1.6 mg-dexamethazone, significantly increased basal biliary output in perfused rat liver.
2.495±0.299 μL/min/g liver dexamethazone group
2.152±0.083 μL/min/g liver control group
P=0.00162
2.Hydrogen sulfide is generated through cysteine metabolism with the catalytic enzyme such as cytathionine γ-lyase (CSE) in the liver. In the rat liver, the content of tissue hydrogen sulfide was around 80 μmol/g tissue, which was firstly measured by our gas-choromatography technique.
3.Propagylglycine (PPG) administrated intraperitoneally, which was a potent inhibitor of CSE, significantly decreased tissue hydrogen sulfide level.
4.The bile output and its constituents were evaluated in both in vivo and ex vivo perfusion system.
(1)In the PPG-treated liver, basal biliary output significantly increased compared with that of control liver.
(2)Supplementation of NaHS in the PPG-treated liver completely inhibited the PPG-effect on bile output in the prefused rat liver.
(3)The elevation of basal bile output in the PPG-treated liver resulted from the increased excretion of biliary bicarbonate. And supplementation of NaHS also inhibited this PPG-effect.
Hydrogen sulfide works as an endogenous gaseous modulator of the basal bile formation in the liver. And it modulates biliary bicarbonate excretion.
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