2004 Fiscal Year Final Research Report Summary
Irradiation for Ex vivo graft to prevent the small-bowel rejection
| Project/Area Number |
15591366
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Nihon University |
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Principal Investigator |
SUGITO Kiminobu Nihon University, Graduate School of medicine, staff, 医学部, 助手 (10328750)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUZAWA Masahiro Osaka University, Graduate School of medicine, Professor, 大学院・医学系研究科, 教授 (60165272)
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| Project Period (FY) |
2003 – 2004
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| Keywords | small bowel transplantation |
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| Research Abstract |
MAdCAM-1 expression on HEVs in PPs was down-regulated during rejection. In contrast its expression on endothelial cells of vessels in the lumina propria was up-regulated during rejection. On day 5 the number of Fas- and FasL-postive enterocytes were increased significantly in the allografts compared with the nontransplanted controls. Similarly, in the PPs, Fas- and FasL-positive mononuclear cells also increased significantly on day 5 in the allografts. Alteration in MAdCAM-1 expression may be associated with the development of SB graft rejection. Activation of fes/FasL system occurred during SB graft rejection suggest that it may be associated with the development of SB graft rejection.
We also studied about the effect of ex vivo graft irradiation and FTY720. Graft survival was prolonged in FTY720 model and ex vivo graft irradiation +FTY720 model. In FTY720 model and ex vivo graft irradiation +FTY720 model, the number of the expression of MAdCAM-1 expression on HEVs in PPs and, Fas/FasL-positive mononuclear cells in the PPs were decreased.
These findings suggest that ex vivo graft irradiation +FTY720 is important for the control of SB graft rejection.
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