2004 Fiscal Year Final Research Report Summary
Biological Effect of Irradiation on Adhesion Molecules in Human Colon Cancer Cells and Application to Clinical Medicine
| Project/Area Number |
15591424
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Nagoya City University |
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Principal Investigator |
SATO Mikinori Nagoya City University, Graduate School of Medical Science, Assistant Professor, 大学院・医学研究科, 講師 (20305551)
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| Co-Investigator(Kenkyū-buntansha) |
MANABE Tadao Nagoya City University, Graduate School of Medical Sciences, Professor, 大学院・医学研究科, 教授 (80127141)
TAKEYAMA Hiromitsu Nagoya City University, Graduate School of Medical Sciences, Associate Professor, 大学院・医学研究科, 助教授 (00216946)
TANAKA Moritsugu Nagoya City University, Graduate School of Medical Sciences, Assistant Professor, 大学院・医学研究科, 講師 (10227184)
SAWAI Hirozumi Nagoya City University, Graduate School of Medical Sciences, Reseach Associate, 大学院・医学研究科, 助手 (40336681)
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| Project Period (FY) |
2003 – 2004
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| Keywords | preonerative radiotherany / sialyl Lewis^a antibody / sialyl Lewis^x antibody / irradiation / liver metastasis / adhesion molecules / colon cancer |
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| Research Abstract |
Nowadays, it is commonly believed that certain cell adhesion molecules play critical roles in cancer metastasis. The adhesion of circulating cancer cells to the vascular endothelium is an important step in the hematogenous metastasis of cancer. Carbohydrate antigens, such as sialyl Lewis^a antigen ( s-Le^a) and sialyl Lewis^x antigen ( s-Le^x), play an important role in cancer metastasis in vitro and in vivo. Especially s-Le^a plays an important role in metastasis to the liver. Currently, preoperative radiotherapy is used for preventing local recurrence of rectal cancer. We investigated the effects of X-ray irradiation on carbohydrate antigens s-Le^a and s-Le^x in vitro.
The cell surface expressions of s-Le^a and s-Le^x were determined by flow cytometric analysis at 24 hours after X-ray irradiation of 4 human colon cancer cell lines. s-Le^a and s-Le^x functions were quantitated using a monolayer cell adhesion assay with human umbilical vein endothelial cells ( HUVECs).
In this study the expression of s-Le^a decreased significantly at 24 hours after any dose of irradiation in HT 29 and WiDr cells. And X-ray irradiation significantly decreased the cell adhesion capacity of HT 29 and WiDr cells to the HUVECs.
These results may indicate the possibility of a reduction of liver metastasis in colon cancer using X-ray irradiation.
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