2004 Fiscal Year Final Research Report Summary
Study for neuronal regeneration in ischemic brain using bone marrow transplantation
| Project/Area Number |
15591555
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Kansai Medical University |
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Principal Investigator |
KAWAMOTO Keiji Kansai Medical University, Faculty of Medicine, Professor, 医学部, 教授 (70077741)
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| Co-Investigator(Kenkyū-buntansha) |
IKEHARA Susumu Kansai Medical University, Faculty of Medicine, Professor, 医学部, 教授 (90108986)
YOSHIMURA Kunikazu Kansai Medical University, Faculty of Medicine, Assistant, 医学部, 助手 (60298891)
HOSAKA Naoki Kansai Medical University, Faculty of Medicine, Assistant Prof, 医学部, 講師 (30388459)
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| Project Period (FY) |
2003 – 2004
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| Keywords | bone marrow / stem cell / transplantation / differentiation / f regeneration |
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| Research Abstract |
The concept of neuronal regeneration has been rapidly changed from the past regenerative medicine. Recently neuronal differentiation and transplantation could be possible using by embryonal stem cell. Regeneration is much interested in the clinical field, so we studied for the possibility of neuronal regeneration in ischemic bran using bone marrow transplantation.
Experiment of ischemic brain
We succeeded in modeling complete occulusion of ipsilatenal middle cerebral artery.
Differentiation of bone marrow stem cells (BMC)
BMS including the hematopoietic and mesenchymal cells could be differentiated into neuronal direction by adding PDGF-BB and/or b-FGF.
Sorting and neuronal expression of CD34
CD34- and CD34+ cells of lineage depleted BMC were sorted and neuronal differentiation showing lin-CD34-adult murine BMC were found by immunocytochemistry and PCR.
Improvement of ischemic brain
The ischemic region was improved by transplantation of BMC.
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