2004 Fiscal Year Final Research Report Summary
Detection of human papilloma virus(HPV) DNA by microdissection of adenocarcinoma of the uterine cervix
| Project/Area Number |
15591728
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
TSUNODA Hajime University of Tsukuba, Graduate School of Comprehensive Human Science, Department Obstetrics and Gynecology, Associate Professor, 大学院・人間総合科学研究科, 助教授 (60197754)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIKAWA Hiroyuki University of Tsukuba, Graduate School of Comprehensive Human Science, Department Obstetrics and Gynecology, Professor, 大学院・人間総合科学研究科, 教授 (40158415)
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| Project Period (FY) |
2003 – 2004
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| Keywords | Cervical adenocarcinoma / Human Papilloma Virus / Laser capture microdissection |
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| Research Abstract |
The aim of this study was to assess the correct ratio of infection of HPV and its subtype of adenocarcinoma itself, using laser capture microdissection method to eliminate the contamination of squamous cells and stromal cells. We analyzed 60 adenocarcinomas of the uterine cervix (19 endometrioid type, 37 endocervical type, 1 intestinal type, 2 poor differentiated type and 1 unclassified adenocarcinoma), and 16 of squamous epithelium around the adenocarcinoma for presence of human papillomavirus and viral subtype using laser capture microdissection method and polymerase chain reaction method.
There are 21 cases with HPV of 37 endocervical types (56.8%), 15 of 19 endometrioid types (78.9%), 1 of 2 poor differentiated types (50.0%), 1 of 1 unclassified type, respectively. And there is no case with HPV in intestinal type. Totally, we found 38 cases with HPV of 60 adenocarcinomas of the cervix (63.3%). There is no case demonstrated different type between adenocarcinoma and CIN around adenocarcinoma. Our studies support that human papillomavirus may play a certain role in the adenocarcinoma pathogenesis of the uterine cervix.
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[Journal Article] Association of PTEN mutation with HPV-negative adenocarcinoma of the uterine cervix.2004
Author(s)
Minaguchi T, Yoshikawa H, Nakagawa S, Yasugi T, Yano T, Iwase H, Mizutani K, Shiromizu K, Ohmi K, Watanabe Y, Noda K, Nishiu M, Nakamura Y, Taketani Y.
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Journal Title
Cancer Lett. 210(1)
Pages: 57-62
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Safety and immunogenicity of a peptide containing the cross-neutralization epitope of HPV16 L2 administered nasally in healthy volunteers.2003
Author(s)
Kawana K, Yasugi T, Kanda T, Kino N, Oda K, Okada S, Kawana Y, Nei T, Takada T, Toyoshima S, Tsuchiya A, Kondo K, Yoshikawa H, Tsutsumi O, Taketani Y.
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Journal Title
Vaccine. 21(27-30)
Pages: 4256-4260
Description
「研究成果報告書概要(欧文)」より
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