2004 Fiscal Year Final Research Report Summary
A laboratory and clinical study of the mechanism of mother-to-child infection of Toxoplasma
| Project/Area Number |
15591732
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TSUTSUMI Osamu The University of Tokyo, Faculty of Medicine, Professor, 医学部附属病院, 教授 (60134574)
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| Project Period (FY) |
2003 – 2004
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| Keywords | Toxoplasma / avidity / anti-Toxoplasma IgM antibody / mother-to-child transmission / congenital toxoplasmosis / murine model / per oral infection / small intestine |
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| Research Abstract |
Objectives
Congenital Toxoplasma infection in the fetus frequently results in debilitating disease in the newborn. To better evaluate the period of maternal infection, we examined avidity of maternal anti-Toxoplasma IgG.
Study Methods
One hundred and forty-four anti-Toxoplasma IgM-positive pregnant women were prospectively tested for IgG avidity for Toxoplasma to determine the period of Toxoplasma infection. We adapted this serologic technique to a commercial ELISA kit "Toxo IgG EIA Seiken" (Denka Seiken, Japan) using 8 M urea as a dissociating agent.
Results
・The mean specific IgM of the pregnant women was 2.65 +/-1.36.
・Of the 144 pregnant women 123 (85.4%) had high avidity results (indicative of previous infection), 9(6.3%) had low avidity results (indicative of recent infection), and 12 (8.3%) had borderline results.
・All of 72 neonates had no physical and neurological abnormality. Three were LFD, 8 were HFD, and 55 were AFD.
・neonates ; 72 cases without anomaly
・positive anti-Toxoplasma IgM ; 0/66
・anti-Toxoplasma IgM : 0.24±0.12 (m±SD)
・abnormal pathological findings of placenta : 0/29
・positive PCR in placenta and cord bloods : 0/16
・Abnormal funduscopic findings : 0/36
・Abnormal CT scan findings : 0/29
・Half-life of anti-Toxoplasma IgG : 31.4±4.5 days (23 non-infected offspring)
Conclusions
As a high IgG avidity can differentiate previously inflected women from IgM-positive pregnant women, measurement of the avidity will avoid unnecessary amniocentesis, medication, and artificial abortion.
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