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2005 Fiscal Year Final Research Report Summary

Functional analysis of LH/hCG receptor in human placental chorionic villous macrophages

Research Project

Project/Area Number 15591763
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionKumamoto University

Principal Investigator

KATABUCHI Hidetaka  Kumamoto University, Faculty of Medical and Pharmaceutical Sciences, Professor, 大学院・医学薬学研究部, 教授 (90224451)

Co-Investigator(Kenkyū-buntansha) OHBA Takashi  Kumamoto University, Faculty of Medical and Pharmaceutical Sciences, Professor, 大学院・医学薬学研究部, 助教授 (50244132)
TASHIRO Hironori  Kumamoto University, University Hospital, Assistant Professor, 医学部附属病院, 講師 (70304996)
Project Period (FY) 2003 – 2005
KeywordsPlacenta / Villi / Macrophage / LH-hCG receptor / exon 9-deleted LH-hCG receptor / THP-1 cell / hCG / hCG β subunit core fragment / hCG / hCGβ subunit core fragment
Research Abstract

Human gonads and non-gonadal organs/tissues express LH/CG receptors. This study aimed to identify the LH/CG receptors and to clarify their function in human placental chorionic villous macrophages. Macrophages as well as syncytiotrophoblasts of human chorionic villous tissues were immunohistochemically positive for LH/CG receptor throughout gestation. By RT-PCR, villous macrophages were shown to express a variant type of LH/CG receptor, the sequencing of which revealed a deletion of exon 9. For experiments in vitro, a monocyte-macrophage cell line, THP-1, was transfected with vector alone, wild-type LH/CG receptor, and exon 9-deleted LH/CG receptor after phorbol 12-myristate 13-acetate (PMA) treatment. Non-PMA-treated THP-1 cells transfected with vector alone were also examined. THP-1 cells expressed exon 9-deleted LH/CG receptor after treatment with PMA. After the cells of the four groups were cultured in medium containing intact hCG, the concentrations of hCG and its b-core fragment … More (β-CF) were measured in the supernatant of the culture medium and in the cell cytosol. Time-dependent hCG uptake was observed in both non-PMA-treated and PMA-treated THP-1 cells, suggesting that the variant receptor is not directly involved in the ingestion of hCG. The degradation of hCG and excretion of β-CF were progressed in PMA-treated cells but not in the un-treated cells. In the cell cytosol, the ratio of β-CF and hCG concentrations (β-CF/hCG) was significantly higher in the PMA-treated cells than in non-PMA-treated cells ; however, it did not differ between the PMA-treated cells transfected with exon 9-deleted receptor and those transfected with vector alone. Macrophages may express the variant receptor in order to recognize the intracytoplasmic hCG and transport it to the lysosome. Among the two PMA-treated cells, the ratio was lower in those transfected with wild-type receptor. The expression of the variant receptor may modulate the degradation of hCG but be reduced by expression of the wild-type receptor in its lacking macrophages. Our data suggest a potentially important role for exon 9-deleted LH/CG receptors expressed in human placental villous macrophages in the local metabolism of hCG. Less

  • Research Products

    (7 results)

All 2006 2005

All Journal Article (7 results)

  • [Journal Article] Characterization of 17beta-hydroxysteroid dehydrogenase type 4 in human ovarian surface epithelial cell.2006

    • Author(s)
      Nagayoshi Y, Ohba T, Yamamoto H, Miyahara Y, Tashiro H, Katabuchi H, et al.
    • Journal Title

      Mol Hum Reprod 11

      Pages: 615-621

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Expression of variant luteinizing hormone/chorionic gonadotropin receptors and degradation of chorionic gonadotropin in human chorionic villous macrophages.2005

    • Author(s)
      Sonoda N, Katabuchi H, Tashiro H et al.
    • Journal Title

      Placenta 26

      Pages: 298-307

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Pathophysiological dynamics of human ovarian surface epithelial cells in epithelial ovarian carcinogenesis.2005

    • Author(s)
      Okamura H, Katabuchi H.
    • Journal Title

      Int Rev Cytol 242

      Pages: 1-54

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Establishment of an immortalised human ovarian surface epithelial cell line without chromosomal instability.2005

    • Author(s)
      Maeda T, Tashiro H, Katabuchi H, et al.
    • Journal Title

      Br J Cancer 93

      Pages: 116-123

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Histologic type,organ of origin,and Wnt pathway status : effect on gene expression in ovarian and uterine carcinomas.2005

    • Author(s)
      Shedden KA, Kshirsagar MP, Schwartz DR, Wu R, Yu H, Misek DE, Hanash S, Katabuchi H, et al.
    • Journal Title

      Clin Cancer Res 11

      Pages: 2123-2131

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Expression of variant luteinizing hormone/chorionic gonadotropin receptors and degradation of chorionic gonadotropin in human chorionic villous macrophages.2005

    • Author(s)
      Sonoda N, Katabuchi H, Tashiro H, et al.
    • Journal Title

      Placenta 26

      Pages: 298-307

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Histologic type, organ of origin, and Wnt pathway status : effect on gene expression in ovarian and uterine carcinomas.2005

    • Author(s)
      Shedden KA, Kshirsagar MP, Schwartz DR, Wu R, Yu H, Misek DE, Hanash S, Katabuchi H, et al.
    • Journal Title

      Clin Cancer Res 11

      Pages: 2123-2131

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2007-12-13  

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