2004 Fiscal Year Final Research Report Summary
Basic Study for Autologous Vaccine Therapy for Neuroblastoma
| Project/Area Number |
15591894
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatric surgery
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| Research Institution | Osaka University |
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Principal Investigator |
FUKUZAWA Masahiro Osaka University, Graduate School of Medicine, Professor, 医学系研究科, 教授 (60165272)
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| Co-Investigator(Kenkyū-buntansha) |
YONEDA Akihiro Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (30372618)
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| Project Period (FY) |
2003 – 2004
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| Keywords | Neuroblastoma / CpG-ODN / apoptosis / GM-CSF / Vaccine Therapy |
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| Research Abstract |
The aim of the study was to clarify the anti-tumor effect of combination vaccine therapy using irradiated autologous neuroblastoma and CpG oligonucleotide with or without GM-CSF which activates dendritic cells, in a mouse model. Also we investigated the effect of this vaccine therapy to minimal residual disease. We inoculated weakly-immunogenic Neuro-2a cells in syngeneic A/J mouse. After resection of the subcutaneous tumor mass, these cells were prepared by irradiation and then used for vaccination. CpG ODN and GM-CSF producing B78H1 cells were injected together with the vaccination. Tumor growth in the vaccination after surgery group was significantly suppressed relative to the control, surgery alone, and vaccination alone groups. Survival was also significantly improved in the vaccination after surgery group.
Although the production of IFN-γ in spleen cells was suppressed by the combination vaccine therapy in a mouse with huge tumor, the production was activated in a mouse whose tumor had been resected. Thus, this combination vaccine therapy was more effective in a mouse with minimal residual disease than in a mouse with huge tumor.
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