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2005 Fiscal Year Final Research Report Summary

Fibronectin participates in the initiation of periodontitis

Research Project

Project/Area Number 15592004
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathobiological dentistry/Dental radiology
Research InstitutionNihon University

Principal Investigator

OHSHIMA Mitsuhiro  Nihon University, School of Dentistry, Lecturer, 歯学部, 講師 (30194145)

Co-Investigator(Kenkyū-buntansha) YAMAGUCHI Yoko  Nihon University, School of Dentistry, Assistant, 歯学部, 副手 (00239922)
OTSUKA Kichibee  Nihon University, School of Dentistry, Professor, 歯学部, 教授 (50059995)
Project Period (FY) 2003 – 2005
Keywordsfibronectin / periodontal ligament fibroblasts / gingival epithelial cells / chemotaxis / integrin
Research Abstract

We examined whether fibronectin produced by periodontal tissue-derived fibroblasts participates in the initiation of periodontitis by its chemotactic activity to a gingival epithelial cell line. The extracellular matrix component, fibronectin, laminin-8/9, and laminin-2/4 secreted by periodontal ligament fibroblasts induced chemotaxis of gingival epithelial cells. To examine RNA integrity during tissue transportation, various conditions were tested. RNA was stable in room temperature for at least 24 h, however, the gene expression profile was stable when the tissue was stored at 4 degrees C examined by real-time PCR. For the accurate diagnosis of periodontal disease, we examined the profiles of cytokine in gingival crevicular fluid using Cytokine Antibody Array. We have found ten novel cytokine, growth factor and its related protein (GRO, Ang, IGFBP-3, GDNF, PARC, OSM, FGF-4, IL-16, PlGF) in gingival crevicular fluid from periodontitis-affected sites. The results suggested that the initiation and progression of periodontal disease may be induced by not only inflammatory cytokine but by various growth factors and its related protein. In the last experiment, we detected that PDGF-B transfected sis-NIH3T3 fibroblasts' spontaneous migration is inhibited by cellular fibronectin.

  • Research Products

    (6 results)

All 2006 2005

All Journal Article (6 results)

  • [Journal Article] Biobanking of fresh frozen tissue : RNA is stable in non-fixed surgical specimens.2006

    • Author(s)
      Micke P, Ohshima M, et al.
    • Journal Title

      Laboratory Investigation 86・2

      Pages: 202-211

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Profiling the cytokines in gingival crevicular fluid using a cytokine antibody array.2006

    • Author(s)
      Sakai A, Ohsima M, et al.
    • Journal Title

      Journal of Periodontology 77・5

      Pages: 856-864

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Biobanking of fresh frozen tissue : RNA is stable in non-fixed surgical specimens.2006

    • Author(s)
      Micke P, Ohshima M, et al.
    • Journal Title

      Laboratory Investigation 86(2)

      Pages: 202-211

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Profiling the cytokines in gingival crevicular fluid using a cytokine antibody array.2006

    • Author(s)
      Sakai S, Ohshima M, et al.
    • Journal Title

      Journal of Periodontology 77(5)

      Pages: 856-864

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Periodontal ligament fibroblasts produce laminin and fibronectin as potent chemoattractants for gingival epithelial cells.2005

    • Author(s)
      Ohshima M, Yamaguchi Y, Otsuka K
    • Journal Title

      International Congress Series 1284

      Pages: 215-216

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Periodontal ligament fibroblasts produce laminin and fibronectin as potent chemoattractant for gingival epithelial cells2005

    • Author(s)
      Ohshima M, Yamaguchi Y, Kichibee Otsuka
    • Journal Title

      International Congress Series 1284

      Pages: 215-216

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2007-12-13  

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