2005 Fiscal Year Final Research Report Summary
Fibronectin participates in the initiation of periodontitis
| Project/Area Number |
15592004
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Nihon University |
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Principal Investigator |
OHSHIMA Mitsuhiro Nihon University, School of Dentistry, Lecturer, 歯学部, 講師 (30194145)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAGUCHI Yoko Nihon University, School of Dentistry, Assistant, 歯学部, 副手 (00239922)
OTSUKA Kichibee Nihon University, School of Dentistry, Professor, 歯学部, 教授 (50059995)
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| Project Period (FY) |
2003 – 2005
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| Keywords | fibronectin / periodontal ligament fibroblasts / gingival epithelial cells / chemotaxis / integrin |
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| Research Abstract |
We examined whether fibronectin produced by periodontal tissue-derived fibroblasts participates in the initiation of periodontitis by its chemotactic activity to a gingival epithelial cell line. The extracellular matrix component, fibronectin, laminin-8/9, and laminin-2/4 secreted by periodontal ligament fibroblasts induced chemotaxis of gingival epithelial cells. To examine RNA integrity during tissue transportation, various conditions were tested. RNA was stable in room temperature for at least 24 h, however, the gene expression profile was stable when the tissue was stored at 4 degrees C examined by real-time PCR. For the accurate diagnosis of periodontal disease, we examined the profiles of cytokine in gingival crevicular fluid using Cytokine Antibody Array. We have found ten novel cytokine, growth factor and its related protein (GRO, Ang, IGFBP-3, GDNF, PARC, OSM, FGF-4, IL-16, PlGF) in gingival crevicular fluid from periodontitis-affected sites. The results suggested that the initiation and progression of periodontal disease may be induced by not only inflammatory cytokine but by various growth factors and its related protein. In the last experiment, we detected that PDGF-B transfected sis-NIH3T3 fibroblasts' spontaneous migration is inhibited by cellular fibronectin.
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