2004 Fiscal Year Final Research Report Summary
Regulation of pathologic root resorption by control of MAPK signaling
| Project/Area Number |
15592169
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
HOTOKEZAKA Hitoshi Nagasaki University, Graduate School of Biomedical Science, Assistant Professor, 大学院・医歯薬学総合研究科, 講師 (90199513)
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| Co-Investigator(Kenkyū-buntansha) |
KITAMURA Akira Nagasaki University, Graduate School of Biomedical Science, Associate Professor, 大学院・医歯薬学総合研究科, 助教授 (30094767)
NEMOTO Takayuki Nagasaki University, Graduate School of Biomedical Science, Professor, 大学院・医歯薬学総合研究科, 教授 (90164665)
NAKAYAMA Koji Nagasaki University, Graduate School of Biomedical Science, Professor, 大学院・医歯薬学総合研究科, 教授 (80150473)
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| Project Period (FY) |
2003 – 2004
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| Keywords | odontoclasts / osteoclasts / root resorption / MAPK / MEK / ERK / p38 / RANKL / differentiation |
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| Research Abstract |
Root resorption sometimes occurs by orthodontic treatment, tooth dislocation, ooclusal interference, reimplantation of tooth, and it is one of big problems in dental treatment. The causative cells are odontoclasts that is basically thought as osteoclasts. In order to investigate the effect of MAPK signaling in root resorption, bone marrow macrophage was cultured in the presence of M-CSF and RANKL on calcium phosphate-coated plate. In this in vitro culture system, MAPK inhibitors were added to see the effect. After fixation and TRAP staining, the number of osteoclasts and pit formation were counted. In the presence of MEK/ERK inhibitors, increase of osteoclast number was observed. On the other hand, p38MAPK suppressed osteoclastogenesis. Then, MEK,ERK,and p38(wild type form and constitutive active form) were transfected into the cell of the culture system. However, the difference was not observed. Then, the time effect of MAPK inhibitors in the osteoclastogenesis was investigated. P38MAPK inhibitor SB203580 inhibited osteoclastogenesis in the early stage of differentiation, and it had no effect in the late stage of osteoclastognesis. Furthermore, in the late stage of osteoclastogenesis, not only RANKL but also other proinflammatory factors such as TNF-alpha, lipopolysaccharide induced fusion of osteoclasts, which implies periodontal inflammation factors enhance root resorption during chronic inflammatory circumstances caused by orthodontic treatment and occlusal trauma
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