マヌカハニー摂取による大腸がん予防機構の解明に向けたニュートリオミクス研究

2016 Fiscal Year Annual Research Report

• Project/Area Number: 15F15395
• Research Institution: Keio University
• Principal Investigator: 冨田 勝 慶應義塾大学, 環境情報学部(藤沢), 教授 (60227626)
• Co-Investigator(Kenkyū-buntansha): AW WANPING 慶應義塾大学, 環境情報学部(藤沢), 外国人特別研究員
• Project Period (FY): 2015-11-09 – 2018-03-31
• Keywords: Rice Bran / Gut microbiota / Metabolome / Metagenome / Multi-omics

Outline of Annual Research Achievements

In this year, we conducted a screening experiment using different food products (Manuka Honey, Rice Bran and Soy Bean) in the animal models that we have created in last year. Unfortunately, Manuka Honey and Soy Bean supplementation did not improve colitis. As such, we aimed to elucidate the molecular mechanisms of Oryza sativa, rice bran (RB) dietary intervention on 2.0% dextran sulfate sodium (DSS)-induced colitis C57BL/6J mice model. Body weight loss, disease activity index, colon length and colon histopathology were improved in RB-fed mice. Time-course microbiome and metabolome results suggest that RB-related alterations of gut environment can prevent colitis via establishing gut homeostasis. Our approach is an important tool in developing new therapeutic applications of RB.

Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

We have proven the rice bran supplementation can suppress colitis progression. In addition, we have also performed comprehensive metabolome and metagenome evaluation on the excised tissues and will publish our findings about the anti-colitic properties of rice bran in 2017.

Strategy for Future Research Activity

We will perform further animal experiment using a DSS-induced colitis mice model and an AOM-DSS-induced colon cancer model using different components of rice bran. In this manner, we can pin point out the specific componenets contributing to the anti-colitic and anti-carcinogenic properties of rice bran.

Research Products

• [Journal Article] Eggshell membrane powder ameliorates intestinal inflammation by facilitating the restitution of epithelial injury and alleviating microbial dysbiosis (2017)
  • Author(s): Huijuan Jia, Manaka Hanate, Wanping Aw, Hideomi Itoh, Kenji Saito, Shoko Kobayashi, Satoshi Hachimura, Shinji Fukuda,Masaru Tomita, Yukio Hasebe, and Hisanori Kato.
  • Journal Title: Scientific Reports Volume: 7 Pages: 43993
  • DOI: 10.1038/srep43993
  • Peer Reviewed / Open Access
• [Journal Article] Understanding the role of the gut ecosystem in Diabetes Mellitus (2017)
  • Author(s): Wanping Aw, Shinji Fukuda
  • Journal Title: Journal of Diabetes Investigation Volume: 印刷中 Pages: 印刷中
  • DOI: 10.1111/jdi.12673
  • Peer Reviewed / Open Access